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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Syntrophobacter fumaroxidans 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP367466SVD
CSB-EP367466SVD
CSB-BP367466SVD
CSB-MP367466SVD
CSB-EP367466SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans 50S ribosomal protein L9 (rplI) CSB-YP367467SVD
CSB-EP367467SVD
CSB-BP367467SVD
CSB-MP367467SVD
CSB-EP367467SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Flavoprotein wrbA (wrbA) CSB-YP367468SVD
CSB-EP367468SVD
CSB-BP367468SVD
CSB-MP367468SVD
CSB-EP367468SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Hydroxylamine reductase (hcp), partial CSB-YP367469SVD
CSB-EP367469SVD
CSB-BP367469SVD
CSB-MP367469SVD
CSB-EP367469SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP367470SVD
CSB-EP367470SVD
CSB-BP367470SVD
CSB-MP367470SVD
CSB-EP367470SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP367471SVD
CSB-EP367471SVD
CSB-BP367471SVD
CSB-MP367471SVD
CSB-EP367471SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans UPF0296 protein Sfum_3631 (Sfum_3631) CSB-YP367472SVD
CSB-EP367472SVD
CSB-BP367472SVD
CSB-MP367472SVD
CSB-EP367472SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans GTPase obg (obg) CSB-YP367473SVD
CSB-EP367473SVD
CSB-BP367473SVD
CSB-MP367473SVD
CSB-EP367473SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP367474SVD
CSB-EP367474SVD
CSB-BP367474SVD
CSB-MP367474SVD
CSB-EP367474SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Ribosomal protein L11 methyltransferase (prmA) CSB-YP367475SVD
CSB-EP367475SVD
CSB-BP367475SVD
CSB-MP367475SVD
CSB-EP367475SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP367476SVD
CSB-EP367476SVD
CSB-BP367476SVD
CSB-MP367476SVD
CSB-EP367476SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans GTP cyclohydrolase folE2 (folE2) CSB-YP367477SVD
CSB-EP367477SVD
CSB-BP367477SVD
CSB-MP367477SVD
CSB-EP367477SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP367478AUA
CSB-EP367478AUA
CSB-BP367478AUA
CSB-MP367478AUA
CSB-EP367478AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP367479AUA
CSB-EP367479AUA
CSB-BP367479AUA
CSB-MP367479AUA
CSB-EP367479AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Aspartate 1-decarboxylase (panD) CSB-YP367480AUA
CSB-EP367480AUA
CSB-BP367480AUA
CSB-MP367480AUA
CSB-EP367480AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP367481AUA
CSB-EP367481AUA
CSB-BP367481AUA
CSB-MP367481AUA
CSB-EP367481AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP367482AUA
CSB-EP367482AUA
CSB-BP367482AUA
CSB-MP367482AUA
CSB-EP367482AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP367483AUA
CSB-EP367483AUA
CSB-BP367483AUA
CSB-MP367483AUA
CSB-EP367483AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus 50S ribosomal protein L10 (rplJ) CSB-YP367484AUA
CSB-EP367484AUA
CSB-BP367484AUA
CSB-MP367484AUA
CSB-EP367484AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus 50S ribosomal protein L23 (rplW) CSB-YP367485AUA
CSB-EP367485AUA
CSB-BP367485AUA
CSB-MP367485AUA
CSB-EP367485AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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