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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coffea arabica NAD (P)H-quinone oxidoreductase subunit 5, chloroplastic, partial CSB-YP366346DVA
CSB-EP366346DVA
CSB-BP366346DVA
CSB-MP366346DVA
CSB-EP366346DVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coffea arabica Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP366347DVA
CSB-EP366347DVA
CSB-BP366347DVA
CSB-MP366347DVA
CSB-EP366347DVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coffea arabica NAD (P)H-quinone oxidoreductase subunit H, chloroplastic CSB-YP366348DVA
CSB-EP366348DVA
CSB-BP366348DVA
CSB-MP366348DVA
CSB-EP366348DVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coffea arabica Putative membrane protein ycf1 (ycf1), partial CSB-YP366349DVA
CSB-EP366349DVA
CSB-BP366349DVA
CSB-MP366349DVA
CSB-EP366349DVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylobates agilis Prolactin-inducible protein homolog (PIP) CSB-YP366350HXV
CSB-EP366350HXV
CSB-BP366350HXV
CSB-MP366350HXV
CSB-EP366350HXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Chromosomal replication initiator protein DnaA (dnaA) CSB-YP366351LLT
CSB-EP366351LLT
CSB-BP366351LLT
CSB-MP366351LLT
CSB-EP366351LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S18 (rpsR) CSB-YP366352LLT
CSB-EP366352LLT
CSB-BP366352LLT
CSB-MP366352LLT
CSB-EP366352LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Putative AgrB-like protein (lwe0039), partial CSB-YP366353LLT1
CSB-EP366353LLT1
CSB-BP366353LLT1
CSB-MP366353LLT1
CSB-EP366353LLT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Adenylosuccinate synthetase (purA) CSB-YP366354LLT
CSB-EP366354LLT
CSB-BP366354LLT
CSB-MP366354LLT
CSB-EP366354LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0213 protein lwe0147 (lwe0147) CSB-YP366355LLT
CSB-EP366355LLT
CSB-BP366355LLT
CSB-MP366355LLT
CSB-EP366355LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP366356LLT
CSB-EP366356LLT
CSB-BP366356LLT
CSB-MP366356LLT
CSB-EP366356LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Bifunctional protein GlmU (glmU) CSB-YP366357LLT
CSB-EP366357LLT
CSB-BP366357LLT
CSB-MP366357LLT
CSB-EP366357LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Peptidyl-tRNA hydrolase (pth) CSB-YP366358LLT
CSB-EP366358LLT
CSB-BP366358LLT
CSB-MP366358LLT
CSB-EP366358LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Type III pantothenate kinase (coaX) CSB-YP366359LLT
CSB-EP366359LLT
CSB-BP366359LLT
CSB-MP366359LLT
CSB-EP366359LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Lysine--tRNA ligase (lysS) CSB-YP366360LLT
CSB-EP366360LLT
CSB-BP366360LLT
CSB-MP366360LLT
CSB-EP366360LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Cysteine--tRNA ligase (cysS) CSB-YP366361LLT
CSB-EP366361LLT
CSB-BP366361LLT
CSB-MP366361LLT
CSB-EP366361LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L11 (rplK) CSB-YP366362LLT
CSB-EP366362LLT
CSB-BP366362LLT
CSB-MP366362LLT
CSB-EP366362LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L7/L12 (rplL) CSB-YP366363LLT
CSB-EP366363LLT
CSB-BP366363LLT
CSB-MP366363LLT
CSB-EP366363LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP366364LLT
CSB-EP366364LLT
CSB-BP366364LLT
CSB-MP366364LLT
CSB-EP366364LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Xylose isomerase (xylA) CSB-YP366365LLT
CSB-EP366365LLT
CSB-BP366365LLT
CSB-MP366365LLT
CSB-EP366365LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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