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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arthrobacter sp. Dihydroxy-acid dehydratase (ilvD), partial CSB-YP367046AVA
CSB-EP367046AVA
CSB-BP367046AVA
CSB-MP367046AVA
CSB-EP367046AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Lipoprotein lpqB (lpqB), partial CSB-YP367047AVA
CSB-EP367047AVA
CSB-BP367047AVA
CSB-MP367047AVA
CSB-EP367047AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Glutamyl-tRNA reductase (hemA) CSB-YP367048AVA
CSB-EP367048AVA
CSB-BP367048AVA
CSB-MP367048AVA
CSB-EP367048AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Carboxylate-amine ligase Arth_2891 (Arth_2891) CSB-YP367049AVA
CSB-EP367049AVA
CSB-BP367049AVA
CSB-MP367049AVA
CSB-EP367049AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP367050AVA
CSB-EP367050AVA
CSB-BP367050AVA
CSB-MP367050AVA
CSB-EP367050AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L17 (rplQ) CSB-YP367051AVA
CSB-EP367051AVA
CSB-BP367051AVA
CSB-MP367051AVA
CSB-EP367051AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 30S ribosomal protein S13 (rpsM) CSB-YP367052AVA
CSB-EP367052AVA
CSB-BP367052AVA
CSB-MP367052AVA
CSB-EP367052AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L30 (rpmD) CSB-YP367053AVA
CSB-EP367053AVA
CSB-BP367053AVA
CSB-MP367053AVA
CSB-EP367053AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L5 (rplE) CSB-YP367054AVA
CSB-EP367054AVA
CSB-BP367054AVA
CSB-MP367054AVA
CSB-EP367054AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 30S ribosomal protein S3 (rpsC) CSB-YP367055AVA
CSB-EP367055AVA
CSB-BP367055AVA
CSB-MP367055AVA
CSB-EP367055AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L3 (rplC) CSB-YP367056AVA
CSB-EP367056AVA
CSB-BP367056AVA
CSB-MP367056AVA
CSB-EP367056AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 30S ribosomal protein S12 (rpsL) CSB-YP367057AVA
CSB-EP367057AVA
CSB-BP367057AVA
CSB-MP367057AVA
CSB-EP367057AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP367058AVA
CSB-EP367058AVA
CSB-BP367058AVA
CSB-MP367058AVA
CSB-EP367058AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. UPF0678 fatty acid-binding protein-like protein Arth_3010 (Arth_3010) CSB-YP367059AVA
CSB-EP367059AVA
CSB-BP367059AVA
CSB-MP367059AVA
CSB-EP367059AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP367060AVA
CSB-EP367060AVA
CSB-BP367060AVA
CSB-MP367060AVA
CSB-EP367060AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. UPF0176 protein Arth_3365 (Arth_3365) CSB-YP367061AVA
CSB-EP367061AVA
CSB-BP367061AVA
CSB-MP367061AVA
CSB-EP367061AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 30S ribosomal protein S14 (rpsN) CSB-YP367062AVA
CSB-EP367062AVA
CSB-BP367062AVA
CSB-MP367062AVA
CSB-EP367062AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 30S ribosomal protein S6 (rpsF) CSB-YP367063AVA
CSB-EP367063AVA
CSB-BP367063AVA
CSB-MP367063AVA
CSB-EP367063AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP367064BPO
CSB-EP367064BPO
CSB-BP367064BPO
CSB-MP367064BPO
CSB-EP367064BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP367065BPO
CSB-EP367065BPO
CSB-BP367065BPO
CSB-MP367065BPO
CSB-EP367065BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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