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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Syntrophobacter fumaroxidans Cysteine--tRNA ligase (cysS) CSB-YP367446SVD
CSB-EP367446SVD
CSB-BP367446SVD
CSB-MP367446SVD
CSB-EP367446SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Octanoyltransferase (lipB) CSB-YP367447SVD
CSB-EP367447SVD
CSB-BP367447SVD
CSB-MP367447SVD
CSB-EP367447SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP367448SVD
CSB-EP367448SVD
CSB-BP367448SVD
CSB-MP367448SVD
CSB-EP367448SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Anthranilate phosphoribosyltransferase (trpD) CSB-YP367449SVD
CSB-EP367449SVD
CSB-BP367449SVD
CSB-MP367449SVD
CSB-EP367449SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Probable potassium transport system protein kup 1 (kup1), partial CSB-YP367450SVD
CSB-EP367450SVD
CSB-BP367450SVD
CSB-MP367450SVD
CSB-EP367450SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans DNA mismatch repair protein mutL (mutL), partial CSB-YP367451SVD
CSB-EP367451SVD
CSB-BP367451SVD
CSB-MP367451SVD
CSB-EP367451SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans NADH-quinone oxidoreductase subunit H 2 (nuoH2), partial CSB-YP367452SVD
CSB-EP367452SVD
CSB-BP367452SVD
CSB-MP367452SVD
CSB-EP367452SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans 7-cyano-7-deazaguanine synthase (queC) CSB-YP367453SVD
CSB-EP367453SVD
CSB-BP367453SVD
CSB-MP367453SVD
CSB-EP367453SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Probable cytosol aminopeptidase (pepA), partial CSB-YP367454SVD
CSB-EP367454SVD
CSB-BP367454SVD
CSB-MP367454SVD
CSB-EP367454SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Aspartate carbamoyltransferase (pyrB) CSB-YP367455SVD
CSB-EP367455SVD
CSB-BP367455SVD
CSB-MP367455SVD
CSB-EP367455SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans UPF0182 protein Sfum_2137 (Sfum_2137), partial CSB-YP367456SVD
CSB-EP367456SVD
CSB-BP367456SVD
CSB-MP367456SVD
CSB-EP367456SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Imidazolonepropionase (hutI) CSB-YP367457SVD
CSB-EP367457SVD
CSB-BP367457SVD
CSB-MP367457SVD
CSB-EP367457SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Alanine--tRNA ligase (alaS), partial CSB-YP367458SVD
CSB-EP367458SVD
CSB-BP367458SVD
CSB-MP367458SVD
CSB-EP367458SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Ribonuclease Y (rny), partial CSB-YP367459SVD
CSB-EP367459SVD
CSB-BP367459SVD
CSB-MP367459SVD
CSB-EP367459SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Putative membrane protein insertion efficiency factor (Sfum_2596) CSB-YP367460SVD
CSB-EP367460SVD
CSB-BP367460SVD
CSB-MP367460SVD
CSB-EP367460SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans tRNA modification GTPase MnmE (mnmE) CSB-YP367461SVD
CSB-EP367461SVD
CSB-BP367461SVD
CSB-MP367461SVD
CSB-EP367461SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Bifunctional protein FolD (folD) CSB-YP367462SVD
CSB-EP367462SVD
CSB-BP367462SVD
CSB-MP367462SVD
CSB-EP367462SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP367463SVD
CSB-EP367463SVD
CSB-BP367463SVD
CSB-MP367463SVD
CSB-EP367463SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP367464SVD
CSB-EP367464SVD
CSB-BP367464SVD
CSB-MP367464SVD
CSB-EP367464SVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Syntrophobacter fumaroxidans Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP367465SVD1
CSB-EP367465SVD1
CSB-BP367465SVD1
CSB-MP367465SVD1
CSB-EP367465SVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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