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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis N utilization substance protein B homolog (nusB) CSB-YP368146BOM
CSB-EP368146BOM
CSB-BP368146BOM
CSB-MP368146BOM
CSB-EP368146BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis L-cystine uptake protein tcyP (BALH_3843), partial CSB-YP368147BOM
CSB-EP368147BOM
CSB-BP368147BOM
CSB-MP368147BOM
CSB-EP368147BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 3 CSB-YP368148BOM
CSB-EP368148BOM
CSB-BP368148BOM
CSB-MP368148BOM
CSB-EP368148BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Chaperone protein DnaK (dnaK), partial CSB-YP368149BOM
CSB-EP368149BOM
CSB-BP368149BOM
CSB-MP368149BOM
CSB-EP368149BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Heat-inducible transcription repressor HrcA (hrcA) CSB-YP368150BOM
CSB-EP368150BOM
CSB-BP368150BOM
CSB-MP368150BOM
CSB-EP368150BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Shikimate dehydrogenase (aroE) CSB-YP368151BOM
CSB-EP368151BOM
CSB-BP368151BOM
CSB-MP368151BOM
CSB-EP368151BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Uridine kinase (udk) CSB-YP368152BOM
CSB-EP368152BOM
CSB-BP368152BOM
CSB-MP368152BOM
CSB-EP368152BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0473 protein BALH_3970 (BALH_3970) CSB-YP368153BOM
CSB-EP368153BOM
CSB-BP368153BOM
CSB-MP368153BOM
CSB-EP368153BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Aspartate--tRNA ligase (aspS), partial CSB-YP368154BOM
CSB-EP368154BOM
CSB-BP368154BOM
CSB-MP368154BOM
CSB-EP368154BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP368155BOM
CSB-EP368155BOM
CSB-BP368155BOM
CSB-MP368155BOM
CSB-EP368155BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis GTPase obg (obg) CSB-YP368156BOM
CSB-EP368156BOM
CSB-BP368156BOM
CSB-MP368156BOM
CSB-EP368156BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Probable septum site-determining protein MinC (minC) CSB-YP368157BOM
CSB-EP368157BOM
CSB-BP368157BOM
CSB-MP368157BOM
CSB-EP368157BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Glutamyl-tRNA reductase (hemA) CSB-YP368158BOM
CSB-EP368158BOM
CSB-BP368158BOM
CSB-MP368158BOM
CSB-EP368158BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Trigger factor (tig) CSB-YP368159BOM
CSB-EP368159BOM
CSB-BP368159BOM
CSB-MP368159BOM
CSB-EP368159BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis MutS2 protein (mutS2), partial CSB-YP368160BOM
CSB-EP368160BOM
CSB-BP368160BOM
CSB-MP368160BOM
CSB-EP368160BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L20 (rplT) CSB-YP368161BOM
CSB-EP368161BOM
CSB-BP368161BOM
CSB-MP368161BOM
CSB-EP368161BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L35 (rpmI) CSB-YP368162BOM
CSB-EP368162BOM
CSB-BP368162BOM
CSB-MP368162BOM
CSB-EP368162BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0756 membrane protein BALH_4179 (BALH_4179), partial CSB-YP368163BOM1
CSB-EP368163BOM1
CSB-BP368163BOM1
CSB-MP368163BOM1
CSB-EP368163BOM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0173 metal-dependent hydrolase BALH_4194 (BALH_4194) CSB-YP368164BOM
CSB-EP368164BOM
CSB-BP368164BOM
CSB-MP368164BOM
CSB-EP368164BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Septation ring formation regulator EzrA (ezrA), partial CSB-YP368165BOM
CSB-EP368165BOM
CSB-BP368165BOM
CSB-MP368165BOM
CSB-EP368165BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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