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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidothermus cellulolyticus Enolase (eno) CSB-YP368786AUA
CSB-EP368786AUA
CSB-BP368786AUA
CSB-MP368786AUA
CSB-EP368786AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus 50S ribosomal protein L9 (rplI) CSB-YP368787AUA
CSB-EP368787AUA
CSB-BP368787AUA
CSB-MP368787AUA
CSB-EP368787AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Ribonuclease P protein component (rnpA) CSB-YP368788AUA
CSB-EP368788AUA
CSB-BP368788AUA
CSB-MP368788AUA
CSB-EP368788AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Ferredoxin--NADP reductase 1 (GFO_0125) CSB-YP368789GEQ
CSB-EP368789GEQ
CSB-BP368789GEQ
CSB-MP368789GEQ
CSB-EP368789GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii 30S ribosomal protein S2 (rpsB) CSB-YP368790GEQ
CSB-EP368790GEQ
CSB-BP368790GEQ
CSB-MP368790GEQ
CSB-EP368790GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Alanine--tRNA ligase (alaS), partial CSB-YP368791GEQ
CSB-EP368791GEQ
CSB-BP368791GEQ
CSB-MP368791GEQ
CSB-EP368791GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP368792GEQ
CSB-EP368792GEQ
CSB-BP368792GEQ
CSB-MP368792GEQ
CSB-EP368792GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Ferredoxin--NADP reductase 2 (GFO_0330) CSB-YP368793GEQ
CSB-EP368793GEQ
CSB-BP368793GEQ
CSB-MP368793GEQ
CSB-EP368793GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP368794GEQ
CSB-EP368794GEQ
CSB-BP368794GEQ
CSB-MP368794GEQ
CSB-EP368794GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP368795GEQ
CSB-EP368795GEQ
CSB-BP368795GEQ
CSB-MP368795GEQ
CSB-EP368795GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii 50S ribosomal protein L35 (rpmI) CSB-YP368796GEQ
CSB-EP368796GEQ
CSB-BP368796GEQ
CSB-MP368796GEQ
CSB-EP368796GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii DNA ligase (ligA), partial CSB-YP368797GEQ
CSB-EP368797GEQ
CSB-BP368797GEQ
CSB-MP368797GEQ
CSB-EP368797GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP368798GEQ
CSB-EP368798GEQ
CSB-BP368798GEQ
CSB-MP368798GEQ
CSB-EP368798GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Catalase-peroxidase (katG), partial CSB-YP368799GEQ
CSB-EP368799GEQ
CSB-BP368799GEQ
CSB-MP368799GEQ
CSB-EP368799GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii 30S ribosomal protein S18 (rpsR) CSB-YP368800GEQ
CSB-EP368800GEQ
CSB-BP368800GEQ
CSB-MP368800GEQ
CSB-EP368800GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Histidine--tRNA ligase (hisS) CSB-YP368801GEQ
CSB-EP368801GEQ
CSB-BP368801GEQ
CSB-MP368801GEQ
CSB-EP368801GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Putative pterin-4-alpha-carbinolamine dehydratase (GFO_1496) CSB-YP368802GEQ
CSB-EP368802GEQ
CSB-BP368802GEQ
CSB-MP368802GEQ
CSB-EP368802GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Chorismate synthase (aroC) CSB-YP368803GEQ
CSB-EP368803GEQ
CSB-BP368803GEQ
CSB-MP368803GEQ
CSB-EP368803GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Elongation factor P (efp) CSB-YP368804GEQ
CSB-EP368804GEQ
CSB-BP368804GEQ
CSB-MP368804GEQ
CSB-EP368804GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii UPF0133 protein GFO_1796 (GFO_1796) CSB-YP368805GEQ
CSB-EP368805GEQ
CSB-BP368805GEQ
CSB-MP368805GEQ
CSB-EP368805GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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