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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arthrobacter sp. Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP368866AVA
CSB-EP368866AVA
CSB-BP368866AVA
CSB-MP368866AVA
CSB-EP368866AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Trigger factor (tig) CSB-YP368867AVA
CSB-EP368867AVA
CSB-BP368867AVA
CSB-MP368867AVA
CSB-EP368867AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. D-alanine--D-alanine ligase (ddl) CSB-YP368868AVA
CSB-EP368868AVA
CSB-BP368868AVA
CSB-MP368868AVA
CSB-EP368868AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Ketol-acid reductoisomerase (ilvC) CSB-YP368869AVA
CSB-EP368869AVA
CSB-BP368869AVA
CSB-MP368869AVA
CSB-EP368869AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. UPF0286 protein Arth_2599 (Arth_2599) CSB-YP368870AVA
CSB-EP368870AVA
CSB-BP368870AVA
CSB-MP368870AVA
CSB-EP368870AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. ATP synthase gamma chain (atpG) CSB-YP368871AVA
CSB-EP368871AVA
CSB-BP368871AVA
CSB-MP368871AVA
CSB-EP368871AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. SsrA-binding protein (smpB) CSB-YP368872AVA
CSB-EP368872AVA
CSB-BP368872AVA
CSB-MP368872AVA
CSB-EP368872AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. UPF0182 protein Arth_2749 (Arth_2749), partial CSB-YP368873AVA
CSB-EP368873AVA
CSB-BP368873AVA
CSB-MP368873AVA
CSB-EP368873AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP368874AVA
CSB-EP368874AVA
CSB-BP368874AVA
CSB-MP368874AVA
CSB-EP368874AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 10 kDa chaperonin (groS) CSB-YP368875AVA
CSB-EP368875AVA
CSB-BP368875AVA
CSB-MP368875AVA
CSB-EP368875AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Formate--tetrahydrofolate ligase (fhs), partial CSB-YP368876AVA
CSB-EP368876AVA
CSB-BP368876AVA
CSB-MP368876AVA
CSB-EP368876AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L13 (rplM) CSB-YP368877AVA
CSB-EP368877AVA
CSB-BP368877AVA
CSB-MP368877AVA
CSB-EP368877AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Translation initiation factor IF-1 (infA) CSB-YP368878AVA
CSB-EP368878AVA
CSB-BP368878AVA
CSB-MP368878AVA
CSB-EP368878AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 30S ribosomal protein S8 (rpsH) CSB-YP368879AVA
CSB-EP368879AVA
CSB-BP368879AVA
CSB-MP368879AVA
CSB-EP368879AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L24 (rplX) CSB-YP368880AVA
CSB-EP368880AVA
CSB-BP368880AVA
CSB-MP368880AVA
CSB-EP368880AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L22 (rplV) CSB-YP368881AVA
CSB-EP368881AVA
CSB-BP368881AVA
CSB-MP368881AVA
CSB-EP368881AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L23 (rplW) CSB-YP368882AVA
CSB-EP368882AVA
CSB-BP368882AVA
CSB-MP368882AVA
CSB-EP368882AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L1 (rplA) CSB-YP368883AVA
CSB-EP368883AVA
CSB-BP368883AVA
CSB-MP368883AVA
CSB-EP368883AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Mycothiol acetyltransferase (mshD) CSB-YP368884AVA
CSB-EP368884AVA
CSB-BP368884AVA
CSB-MP368884AVA
CSB-EP368884AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Acetate kinase (ackA) CSB-YP368885AVA
CSB-EP368885AVA
CSB-BP368885AVA
CSB-MP368885AVA
CSB-EP368885AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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