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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. novicida Ribosome maturation factor RimP (rimP) CSB-YP370927FDW
CSB-EP370927FDW
CSB-BP370927FDW
CSB-MP370927FDW
CSB-EP370927FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP370928FDW
CSB-EP370928FDW
CSB-BP370928FDW
CSB-MP370928FDW
CSB-EP370928FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP370929FDW
CSB-EP370929FDW
CSB-BP370929FDW
CSB-MP370929FDW
CSB-EP370929FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida GTPase obg (obg) CSB-YP370930FDW
CSB-EP370930FDW
CSB-BP370930FDW
CSB-MP370930FDW
CSB-EP370930FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Tryptophan synthase beta chain (trpB) CSB-YP370931FDW
CSB-EP370931FDW
CSB-BP370931FDW
CSB-MP370931FDW
CSB-EP370931FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Na (+)/H (+) antiporter nhaA, partial CSB-YP370932FDW
CSB-EP370932FDW
CSB-BP370932FDW
CSB-MP370932FDW
CSB-EP370932FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium UPF0301 protein MAV_0052 (MAV_0052) CSB-YP370933MOI
CSB-EP370933MOI
CSB-BP370933MOI
CSB-MP370933MOI
CSB-EP370933MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Putative regulator of ribonuclease activity (MAV_0174) CSB-YP370934MOI
CSB-EP370934MOI
CSB-BP370934MOI
CSB-MP370934MOI
CSB-EP370934MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Putative phenylalanine aminotransferase (pat) CSB-YP370935MOI
CSB-EP370935MOI
CSB-BP370935MOI
CSB-MP370935MOI
CSB-EP370935MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Recombination protein RecR (recR) CSB-YP370936MOI
CSB-EP370936MOI
CSB-BP370936MOI
CSB-MP370936MOI
CSB-EP370936MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Type III pantothenate kinase (coaX) CSB-YP370937MOI
CSB-EP370937MOI
CSB-BP370937MOI
CSB-MP370937MOI
CSB-EP370937MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Uncharacterized tRNA/rRNA methyltransferase MAV_0574 (MAV_0574) CSB-YP370938MOI
CSB-EP370938MOI
CSB-BP370938MOI
CSB-MP370938MOI
CSB-EP370938MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Acetaldehyde dehydrogenase (MAV_0626) CSB-YP370939MOI
CSB-EP370939MOI
CSB-BP370939MOI
CSB-MP370939MOI
CSB-EP370939MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Trehalose-phosphate synthase (otsA) CSB-YP370940MOI
CSB-EP370940MOI
CSB-BP370940MOI
CSB-MP370940MOI
CSB-EP370940MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium UPF0678 fatty acid-binding protein-like protein MAV_0753 (MAV_0753) CSB-YP370941MOI
CSB-EP370941MOI
CSB-BP370941MOI
CSB-MP370941MOI
CSB-EP370941MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium 3- (3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, partial CSB-YP370942MOI
CSB-EP370942MOI
CSB-BP370942MOI
CSB-MP370942MOI
CSB-EP370942MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP370943MOI
CSB-EP370943MOI
CSB-BP370943MOI
CSB-MP370943MOI
CSB-EP370943MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Arginine deiminase (arcA) CSB-YP370944MOI
CSB-EP370944MOI
CSB-BP370944MOI
CSB-MP370944MOI
CSB-EP370944MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Peptidyl-tRNA hydrolase (pth) CSB-YP370945MOI
CSB-EP370945MOI
CSB-BP370945MOI
CSB-MP370945MOI
CSB-EP370945MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Potassium-transporting ATPase A chain (kdpA), partial CSB-YP370946MOI
CSB-EP370946MOI
CSB-BP370946MOI
CSB-MP370946MOI
CSB-EP370946MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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