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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phaeodactylum tricornutum Photosystem I reaction center subunit XII (psaM), partial CSB-YP371386EUF
CSB-EP371386EUF
CSB-BP371386EUF
CSB-MP371386EUF
CSB-EP371386EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L12, chloroplastic (rpl12) CSB-YP371387EUF
CSB-EP371387EUF
CSB-BP371387EUF
CSB-MP371387EUF
CSB-EP371387EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L11, chloroplastic (rpl11) CSB-YP371388EUF
CSB-EP371388EUF
CSB-BP371388EUF
CSB-MP371388EUF
CSB-EP371388EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Cytochrome c-550 (psbV) CSB-YP371389EUF
CSB-EP371389EUF
CSB-BP371389EUF
CSB-MP371389EUF
CSB-EP371389EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Apocytochrome f (petA), partial CSB-YP371390EUF1
CSB-EP371390EUF1
CSB-BP371390EUF1
CSB-MP371390EUF1
CSB-EP371390EUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP371391EUF
CSB-EP371391EUF
CSB-BP371391EUF
CSB-MP371391EUF
CSB-EP371391EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP371392EUF
CSB-EP371392EUF
CSB-BP371392EUF
CSB-MP371392EUF
CSB-EP371392EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP371393EUF
CSB-EP371393EUF
CSB-BP371393EUF
CSB-MP371393EUF
CSB-EP371393EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP371395EUF
CSB-EP371395EUF
CSB-BP371395EUF
CSB-MP371395EUF
CSB-EP371395EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L35, chloroplastic (rpl35) CSB-YP371396EUF
CSB-EP371396EUF
CSB-BP371396EUF
CSB-MP371396EUF
CSB-EP371396EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Photosystem II protein Y (psbY-A), partial CSB-YP371397EUF
CSB-EP371397EUF
CSB-BP371397EUF
CSB-MP371397EUF
CSB-EP371397EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Protein translocase subunit SecA (secA), partial CSB-YP371398EUF
CSB-EP371398EUF
CSB-BP371398EUF
CSB-MP371398EUF
CSB-EP371398EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Photosystem Q (B) protein, partial CSB-YP371399EUF1
CSB-EP371399EUF1
CSB-BP371399EUF1
CSB-MP371399EUF1
CSB-EP371399EUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Photosystem II reaction center psb28 protein (psb28) CSB-YP371400EUF
CSB-EP371400EUF
CSB-BP371400EUF
CSB-MP371400EUF
CSB-EP371400EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Chaperone protein dnaK (dnaK), partial CSB-YP371401EUF
CSB-EP371401EUF
CSB-BP371401EUF
CSB-MP371401EUF
CSB-EP371401EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L23, chloroplastic (rpl23) CSB-YP371402EUF
CSB-EP371402EUF
CSB-BP371402EUF
CSB-MP371402EUF
CSB-EP371402EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP371403EUF
CSB-EP371403EUF
CSB-BP371403EUF
CSB-MP371403EUF
CSB-EP371403EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP371404EUF
CSB-EP371404EUF
CSB-BP371404EUF
CSB-MP371404EUF
CSB-EP371404EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP371405EUF
CSB-EP371405EUF
CSB-BP371405EUF
CSB-MP371405EUF
CSB-EP371405EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum 50S ribosomal protein L5, chloroplastic (rpl5) CSB-YP371406EUF
CSB-EP371406EUF
CSB-BP371406EUF
CSB-MP371406EUF
CSB-EP371406EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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