Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium smegmatis Iron (II)-dependent oxidoreductase EgtB CSB-YP369887MVX
CSB-EP369887MVX
CSB-BP369887MVX
CSB-MP369887MVX
CSB-EP369887MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Recombination protein RecR (recR) CSB-YP369888MVX
CSB-EP369888MVX
CSB-BP369888MVX
CSB-MP369888MVX
CSB-EP369888MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Catalase-peroxidase 1 (katG1), partial CSB-YP369889MVX
CSB-EP369889MVX
CSB-BP369889MVX
CSB-MP369889MVX
CSB-EP369889MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Putative Rieske 2Fe-2S iron-sulfur protein MSMEG_6410 (MSMEG_6410), partial CSB-YP369890MVX
CSB-EP369890MVX
CSB-BP369890MVX
CSB-MP369890MVX
CSB-EP369890MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis HTH-type transcriptional regulator EthR (ethR) CSB-YP369891MVX
CSB-EP369891MVX
CSB-BP369891MVX
CSB-MP369891MVX
CSB-EP369891MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Maltokinase (pep2) CSB-YP369892MVX
CSB-EP369892MVX
CSB-BP369892MVX
CSB-MP369892MVX
CSB-EP369892MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis UPF0678 fatty acid-binding protein-like protein MSMEG_6574 (MSMEG_6574) CSB-YP369893MVX
CSB-EP369893MVX
CSB-BP369893MVX
CSB-MP369893MVX
CSB-EP369893MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis 30S ribosomal protein S18 2 (rpsR2) CSB-YP369894MVX
CSB-EP369894MVX
CSB-BP369894MVX
CSB-MP369894MVX
CSB-EP369894MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Leucine--tRNA ligase (leuS), partial CSB-YP369895MVX
CSB-EP369895MVX
CSB-BP369895MVX
CSB-MP369895MVX
CSB-EP369895MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP369896MVX
CSB-EP369896MVX
CSB-BP369896MVX
CSB-MP369896MVX
CSB-EP369896MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DNA replication and repair protein recF (recF) CSB-YP369897BOM
CSB-EP369897BOM
CSB-BP369897BOM
CSB-MP369897BOM
CSB-EP369897BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Bifunctional protein GlmU (glmU) CSB-YP369898BOM
CSB-EP369898BOM
CSB-BP369898BOM
CSB-MP369898BOM
CSB-EP369898BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 33 kDa chaperonin (hslO) CSB-YP369899BOM
CSB-EP369899BOM
CSB-BP369899BOM
CSB-MP369899BOM
CSB-EP369899BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP369900BOM
CSB-EP369900BOM
CSB-BP369900BOM
CSB-MP369900BOM
CSB-EP369900BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Cysteine--tRNA ligase (cysS) CSB-YP369901BOM
CSB-EP369901BOM
CSB-BP369901BOM
CSB-MP369901BOM
CSB-EP369901BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP369902BOM
CSB-EP369902BOM
CSB-BP369902BOM
CSB-MP369902BOM
CSB-EP369902BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 30S ribosomal protein S7 (rpsG) CSB-YP369903BOM
CSB-EP369903BOM
CSB-BP369903BOM
CSB-MP369903BOM
CSB-EP369903BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L4 (rplD) CSB-YP369904BOM
CSB-EP369904BOM
CSB-BP369904BOM
CSB-MP369904BOM
CSB-EP369904BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L22 (rplV) CSB-YP369905BOM
CSB-EP369905BOM
CSB-BP369905BOM
CSB-MP369905BOM
CSB-EP369905BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 50S ribosomal protein L14 (rplN) CSB-YP369906BOM
CSB-EP369906BOM
CSB-BP369906BOM
CSB-MP369906BOM
CSB-EP369906BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>