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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Probable crotonobetaine/carnitine-CoA ligase (caiC) CSB-YP371689EJE
CSB-EP371689EJE
CSB-BP371689EJE
CSB-MP371689EJE
CSB-EP371689EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotonobetainyl-CoA dehydrogenase (caiA) CSB-YP371690EJE
CSB-EP371690EJE
CSB-BP371690EJE
CSB-MP371690EJE
CSB-EP371690EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein fixB (fixB) CSB-YP371691EJE
CSB-EP371691EJE
CSB-BP371691EJE
CSB-MP371691EJE
CSB-EP371691EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP371692EJE
CSB-EP371692EJE
CSB-BP371692EJE
CSB-MP371692EJE
CSB-EP371692EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP371693EJE
CSB-EP371693EJE
CSB-BP371693EJE
CSB-MP371693EJE
CSB-EP371693EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase-associated protein RapA (rapA), partial CSB-YP371694EJE
CSB-EP371694EJE
CSB-BP371694EJE
CSB-MP371694EJE
CSB-EP371694EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulokinase (araB) CSB-YP371695EJE
CSB-EP371695EJE
CSB-BP371695EJE
CSB-MP371695EJE
CSB-EP371695EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP371696EJE
CSB-EP371696EJE
CSB-BP371696EJE
CSB-MP371696EJE
CSB-EP371696EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-isopropylmalate synthase (leuA) CSB-YP371697EJE
CSB-EP371697EJE
CSB-BP371697EJE
CSB-MP371697EJE
CSB-EP371697EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP371698EJE
CSB-EP371698EJE
CSB-BP371698EJE
CSB-MP371698EJE
CSB-EP371698EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP371699EJE
CSB-EP371699EJE
CSB-BP371699EJE
CSB-MP371699EJE
CSB-EP371699EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Secretion monitor (secM) CSB-YP371700EJE
CSB-EP371700EJE
CSB-BP371700EJE
CSB-MP371700EJE
CSB-EP371700EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0289 protein yacF (yacF) CSB-YP371701EJE
CSB-EP371701EJE
CSB-BP371701EJE
CSB-MP371701EJE
CSB-EP371701EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantothenate synthetase (panC) CSB-YP371702EJE
CSB-EP371702EJE
CSB-BP371702EJE
CSB-MP371702EJE
CSB-EP371702EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sugar fermentation stimulation protein A (sfsA) CSB-YP371703EJE
CSB-EP371703EJE
CSB-BP371703EJE
CSB-MP371703EJE
CSB-EP371703EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP371704EJE
CSB-EP371704EJE
CSB-BP371704EJE
CSB-MP371704EJE
CSB-EP371704EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP371705EJE
CSB-EP371705EJE
CSB-BP371705EJE
CSB-MP371705EJE
CSB-EP371705EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyguanosinetriphosphate triphosphohydrolase (dgt) CSB-YP371706EJE
CSB-EP371706EJE
CSB-BP371706EJE
CSB-MP371706EJE
CSB-EP371706EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP371707EJE
CSB-EP371707EJE
CSB-BP371707EJE
CSB-MP371707EJE
CSB-EP371707EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S2 (rpsB) CSB-YP371708EJE
CSB-EP371708EJE
CSB-BP371708EJE
CSB-MP371708EJE
CSB-EP371708EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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