Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Campylobacter fetus subsp. fetus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP371326CWC
CSB-EP371326CWC
CSB-BP371326CWC
CSB-MP371326CWC
CSB-EP371326CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus 50S ribosomal protein L11 (rplK) CSB-YP371327CWC
CSB-EP371327CWC
CSB-BP371327CWC
CSB-MP371327CWC
CSB-EP371327CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP371328CWC
CSB-EP371328CWC
CSB-BP371328CWC
CSB-MP371328CWC
CSB-EP371328CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus 2-isopropylmalate synthase (leuA), partial CSB-YP371329CWC
CSB-EP371329CWC
CSB-BP371329CWC
CSB-MP371329CWC
CSB-EP371329CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP371330CWC
CSB-EP371330CWC
CSB-BP371330CWC
CSB-MP371330CWC
CSB-EP371330CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Pantothenate synthetase (panC) CSB-YP371331CWC
CSB-EP371331CWC
CSB-BP371331CWC
CSB-MP371331CWC
CSB-EP371331CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP371332CWC
CSB-EP371332CWC
CSB-BP371332CWC
CSB-MP371332CWC
CSB-EP371332CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP371333CWC
CSB-EP371333CWC
CSB-BP371333CWC
CSB-MP371333CWC
CSB-EP371333CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP371334CWC
CSB-EP371334CWC
CSB-BP371334CWC
CSB-MP371334CWC
CSB-EP371334CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP371335CWC
CSB-EP371335CWC
CSB-BP371335CWC
CSB-MP371335CWC
CSB-EP371335CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Elongation factor 4 (lepA), partial CSB-YP371336CWC
CSB-EP371336CWC
CSB-BP371336CWC
CSB-MP371336CWC
CSB-EP371336CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP371337CWC
CSB-EP371337CWC
CSB-BP371337CWC
CSB-MP371337CWC
CSB-EP371337CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus GTPase obg (obg) CSB-YP371338CWC
CSB-EP371338CWC
CSB-BP371338CWC
CSB-MP371338CWC
CSB-EP371338CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus 50S ribosomal protein L21 (rplU) CSB-YP371339CWC
CSB-EP371339CWC
CSB-BP371339CWC
CSB-MP371339CWC
CSB-EP371339CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus L-seryl-tRNA (Sec) selenium transferase CSB-YP371340CWC
CSB-EP371340CWC
CSB-BP371340CWC
CSB-MP371340CWC
CSB-EP371340CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Deoxycytidine triphosphate deaminase (dcd) CSB-YP371341CWC
CSB-EP371341CWC
CSB-BP371341CWC
CSB-MP371341CWC
CSB-EP371341CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus GTP cyclohydrolase-2 (ribA) CSB-YP371342CWC
CSB-EP371342CWC
CSB-BP371342CWC
CSB-MP371342CWC
CSB-EP371342CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Probable endonuclease 4 (nfo) CSB-YP371343CWC
CSB-EP371343CWC
CSB-BP371343CWC
CSB-MP371343CWC
CSB-EP371343CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Phosphoglucosamine mutase (glmM) CSB-YP371344CWC
CSB-EP371344CWC
CSB-BP371344CWC
CSB-MP371344CWC
CSB-EP371344CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter fetus subsp. fetus Chorismate synthase (aroC) CSB-YP371345CWC
CSB-EP371345CWC
CSB-BP371345CWC
CSB-MP371345CWC
CSB-EP371345CWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>