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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neosartorya fischeri Probable arabinogalactan endo-1,4-beta-galactosidase A (galA) CSB-YP373128NEZ
CSB-EP373128NEZ
CSB-BP373128NEZ
CSB-MP373128NEZ
CSB-EP373128NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Protein sds23 (sds23), partial CSB-YP373129NEZ
CSB-EP373129NEZ
CSB-BP373129NEZ
CSB-MP373129NEZ
CSB-EP373129NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Putative galacturan 1,4-alpha-galacturonidase C (rgxC) CSB-YP373130NEZ
CSB-EP373130NEZ
CSB-BP373130NEZ
CSB-MP373130NEZ
CSB-EP373130NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Small COPII coat GTPase sar1 (sar1) CSB-YP373131NEZ
CSB-EP373131NEZ
CSB-BP373131NEZ
CSB-MP373131NEZ
CSB-EP373131NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Probable xyloglucan-specific endo-beta-1,4-glucanase A (xgeA) CSB-YP373132NEZ
CSB-EP373132NEZ
CSB-BP373132NEZ
CSB-MP373132NEZ
CSB-EP373132NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Polyadenylate-binding protein, cytoplasmic and nuclear (pab1), partial CSB-YP373133NEZ
CSB-EP373133NEZ
CSB-BP373133NEZ
CSB-MP373133NEZ
CSB-EP373133NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cation/acetate symporter ActP (actP), partial CSB-YP373134EJE
CSB-EP373134EJE
CSB-BP373134EJE
CSB-MP373134EJE
CSB-EP373134EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP373135EJE
CSB-EP373135EJE
CSB-BP373135EJE
CSB-MP373135EJE
CSB-EP373135EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein mutL (mutL) CSB-YP373136EJE
CSB-EP373136EJE
CSB-BP373136EJE
CSB-MP373136EJE
CSB-EP373136EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator UlaR (ulaR) CSB-YP373137EJE
CSB-EP373137EJE
CSB-BP373137EJE
CSB-MP373137EJE
CSB-EP373137EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Primosomal replication protein n (priB) CSB-YP373138EJE
CSB-EP373138EJE
CSB-BP373138EJE
CSB-MP373138EJE
CSB-EP373138EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP373139EJE
CSB-EP373139EJE
CSB-BP373139EJE
CSB-MP373139EJE
CSB-EP373139EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cytosol aminopeptidase (pepA) CSB-YP373140EJE
CSB-EP373140EJE
CSB-BP373140EJE
CSB-MP373140EJE
CSB-EP373140EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yjiK (yjiK), partial CSB-YP373141EJE1
CSB-EP373141EJE1
CSB-BP373141EJE1
CSB-MP373141EJE1
CSB-EP373141EJE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0442 protein yjjB (yjjB), partial CSB-YP373142EJE1
CSB-EP373142EJE1
CSB-BP373142EJE1
CSB-MP373142EJE1
CSB-EP373142EJE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 3 (prfC) CSB-YP373143EJE
CSB-EP373143EJE
CSB-BP373143EJE
CSB-MP373143EJE
CSB-EP373143EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trp operon repressor (trpR) CSB-YP373144EJE
CSB-EP373144EJE
CSB-BP373144EJE
CSB-MP373144EJE
CSB-EP373144EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Cysteine--tRNA ligase (cysS) CSB-YP373145PYF
CSB-EP373145PYF
CSB-BP373145PYF
CSB-MP373145PYF
CSB-EP373145PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Recombination protein RecR (recR) CSB-YP373146PYF
CSB-EP373146PYF
CSB-BP373146PYF
CSB-MP373146PYF
CSB-EP373146PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L21 (rplU) CSB-YP373147PYF
CSB-EP373147PYF
CSB-BP373147PYF
CSB-MP373147PYF
CSB-EP373147PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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