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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelobacter propionicus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP373168PYF
CSB-EP373168PYF
CSB-BP373168PYF
CSB-MP373168PYF
CSB-EP373168PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Cobalt transport protein CbiM 1 (cbim1), partial CSB-YP373169PYF1
CSB-EP373169PYF1
CSB-BP373169PYF1
CSB-MP373169PYF1
CSB-EP373169PYF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus N utilization substance protein B homolog (nusB) CSB-YP373170PYF
CSB-EP373170PYF
CSB-BP373170PYF
CSB-MP373170PYF
CSB-EP373170PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus SsrA-binding protein (smpB) CSB-YP373171PYF
CSB-EP373171PYF
CSB-BP373171PYF
CSB-MP373171PYF
CSB-EP373171PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Histidine--tRNA ligase (hisS) CSB-YP373172PYF
CSB-EP373172PYF
CSB-BP373172PYF
CSB-MP373172PYF
CSB-EP373172PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Protein grpE (grpE) CSB-YP373173PYF
CSB-EP373173PYF
CSB-BP373173PYF
CSB-MP373173PYF
CSB-EP373173PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Transcriptional repressor NrdR (nrdR) CSB-YP373174PYF
CSB-EP373174PYF
CSB-BP373174PYF
CSB-MP373174PYF
CSB-EP373174PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Enolase (eno) CSB-YP373175PYF
CSB-EP373175PYF
CSB-BP373175PYF
CSB-MP373175PYF
CSB-EP373175PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Phosphoglycerate kinase (pgk) CSB-YP373176PYF
CSB-EP373176PYF
CSB-BP373176PYF
CSB-MP373176PYF
CSB-EP373176PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Leucine--tRNA ligase (leuS), partial CSB-YP373177PYF
CSB-EP373177PYF
CSB-BP373177PYF
CSB-MP373177PYF
CSB-EP373177PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP373178PYF
CSB-EP373178PYF
CSB-BP373178PYF
CSB-MP373178PYF
CSB-EP373178PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 3-dehydroquinate synthase (aroB) CSB-YP373179PYF
CSB-EP373179PYF
CSB-BP373179PYF
CSB-MP373179PYF
CSB-EP373179PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Uridylate kinase (pyrH) CSB-YP373180PYF
CSB-EP373180PYF
CSB-BP373180PYF
CSB-MP373180PYF
CSB-EP373180PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Phosphoglucosamine mutase (glmM) CSB-YP373181PYF
CSB-EP373181PYF
CSB-BP373181PYF
CSB-MP373181PYF
CSB-EP373181PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L20 (rplT) CSB-YP373182PYF
CSB-EP373182PYF
CSB-BP373182PYF
CSB-MP373182PYF
CSB-EP373182PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP373183PYF
CSB-EP373183PYF
CSB-BP373183PYF
CSB-MP373183PYF
CSB-EP373183PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L28 (rpmB) CSB-YP373184PYF
CSB-EP373184PYF
CSB-BP373184PYF
CSB-MP373184PYF
CSB-EP373184PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Transcription elongation factor GreA (greA) CSB-YP373185PYF
CSB-EP373185PYF
CSB-BP373185PYF
CSB-MP373185PYF
CSB-EP373185PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Ketol-acid reductoisomerase (ilvC) CSB-YP373186PYF
CSB-EP373186PYF
CSB-BP373186PYF
CSB-MP373186PYF
CSB-EP373186PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP373187PYF
CSB-EP373187PYF
CSB-BP373187PYF
CSB-MP373187PYF
CSB-EP373187PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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