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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Paracoccus denitrificans Zinc import ATP-binding protein ZnuC (znuC) CSB-YP372888PYC
CSB-EP372888PYC
CSB-BP372888PYC
CSB-MP372888PYC
CSB-EP372888PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 50S ribosomal protein L33 (rpmG) CSB-YP372889PYC
CSB-EP372889PYC
CSB-BP372889PYC
CSB-MP372889PYC
CSB-EP372889PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Cell division protein FtsQ (ftsQ), partial CSB-YP372890PYC
CSB-EP372890PYC
CSB-BP372890PYC
CSB-MP372890PYC
CSB-EP372890PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Homoserine O-succinyltransferase (metA) CSB-YP372891PYC
CSB-EP372891PYC
CSB-BP372891PYC
CSB-MP372891PYC
CSB-EP372891PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 30S ribosomal protein S20 (rpsT) CSB-YP372892PYC
CSB-EP372892PYC
CSB-BP372892PYC
CSB-MP372892PYC
CSB-EP372892PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans C4-dicarboxylate transport protein (dctA), partial CSB-YP372893PYC
CSB-EP372893PYC
CSB-BP372893PYC
CSB-MP372893PYC
CSB-EP372893PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP372894CWO
CSB-EP372894CWO
CSB-BP372894CWO
CSB-MP372894CWO
CSB-EP372894CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Biotin synthase (bioB) CSB-YP372895CWO
CSB-EP372895CWO
CSB-BP372895CWO
CSB-MP372895CWO
CSB-EP372895CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Enolase (eno) CSB-YP372896CWO
CSB-EP372896CWO
CSB-BP372896CWO
CSB-MP372896CWO
CSB-EP372896CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L7/L12 (rplL) CSB-YP372897CWO
CSB-EP372897CWO
CSB-BP372897CWO
CSB-MP372897CWO
CSB-EP372897CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP372898CWO
CSB-EP372898CWO
CSB-BP372898CWO
CSB-MP372898CWO
CSB-EP372898CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Histidine--tRNA ligase (hisS) CSB-YP372899CWO
CSB-EP372899CWO
CSB-BP372899CWO
CSB-MP372899CWO
CSB-EP372899CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S15 (rpsO) CSB-YP372900CWO
CSB-EP372900CWO
CSB-BP372900CWO
CSB-MP372900CWO
CSB-EP372900CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP372901CWO
CSB-EP372901CWO
CSB-BP372901CWO
CSB-MP372901CWO
CSB-EP372901CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides DNA ligase (ligA), partial CSB-YP372902CWO
CSB-EP372902CWO
CSB-BP372902CWO
CSB-MP372902CWO
CSB-EP372902CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP372903CWO
CSB-EP372903CWO
CSB-BP372903CWO
CSB-MP372903CWO
CSB-EP372903CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Indole-3-glycerol phosphate synthase (trpC) CSB-YP372904CWO
CSB-EP372904CWO
CSB-BP372904CWO
CSB-MP372904CWO
CSB-EP372904CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP372905CWO
CSB-EP372905CWO
CSB-BP372905CWO
CSB-MP372905CWO
CSB-EP372905CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP372906CWO
CSB-EP372906CWO
CSB-BP372906CWO
CSB-MP372906CWO
CSB-EP372906CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP372907CWO
CSB-EP372907CWO
CSB-BP372907CWO
CSB-MP372907CWO
CSB-EP372907CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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