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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0263 protein YE2228 (YE2228) CSB-YP374288YAK
CSB-EP374288YAK
CSB-BP374288YAK
CSB-MP374288YAK
CSB-EP374288YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0225 protein YE2246 (YE2246) CSB-YP374289YAK
CSB-EP374289YAK
CSB-BP374289YAK
CSB-MP374289YAK
CSB-EP374289YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Glutamine--tRNA ligase (glnS), partial CSB-YP374290YAK
CSB-EP374290YAK
CSB-BP374290YAK
CSB-MP374290YAK
CSB-EP374290YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0229 protein YE2273 (YE2273) CSB-YP374291YAK
CSB-EP374291YAK
CSB-BP374291YAK
CSB-MP374291YAK
CSB-EP374291YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Potassium-transporting ATPase C chain (kdpC), partial CSB-YP374292YAK
CSB-EP374292YAK
CSB-BP374292YAK
CSB-MP374292YAK
CSB-EP374292YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP374293YAK
CSB-EP374293YAK
CSB-BP374293YAK
CSB-MP374293YAK
CSB-EP374293YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Multifunctional CCA protein (cca) CSB-YP374294YAK
CSB-EP374294YAK
CSB-BP374294YAK
CSB-MP374294YAK
CSB-EP374294YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP374295YAK
CSB-EP374295YAK
CSB-BP374295YAK
CSB-MP374295YAK
CSB-EP374295YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP374296YAK
CSB-EP374296YAK
CSB-BP374296YAK
CSB-MP374296YAK
CSB-EP374296YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Serine/threonine transporter sstT (sstT), partial CSB-YP374297YAK
CSB-EP374297YAK
CSB-BP374297YAK
CSB-MP374297YAK
CSB-EP374297YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Uronate isomerase (uxaC) CSB-YP374298YAK
CSB-EP374298YAK
CSB-BP374298YAK
CSB-MP374298YAK
CSB-EP374298YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B DnaA initiator-associating protein diaA (diaA) CSB-YP374299YAK
CSB-EP374299YAK
CSB-BP374299YAK
CSB-MP374299YAK
CSB-EP374299YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0745 protein YE2368 (YE2368) CSB-YP374300YAK
CSB-EP374300YAK
CSB-BP374300YAK
CSB-MP374300YAK
CSB-EP374300YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0042 nucleotide-binding protein YE3762 (YE3762) CSB-YP374301YAK
CSB-EP374301YAK
CSB-BP374301YAK
CSB-MP374301YAK
CSB-EP374301YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Spermidine export protein MdtJ (mdtJ), partial CSB-YP374302YAK1
CSB-EP374302YAK1
CSB-BP374302YAK1
CSB-MP374302YAK1
CSB-EP374302YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Dipeptide and tripeptide permease B (dtpB), partial CSB-YP374303YAK
CSB-EP374303YAK
CSB-BP374303YAK
CSB-MP374303YAK
CSB-EP374303YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 3-dehydroquinate dehydratase (aroQ) CSB-YP374304YAK
CSB-EP374304YAK
CSB-BP374304YAK
CSB-MP374304YAK
CSB-EP374304YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Probable transcriptional regulatory protein YE2395 (YE2395) CSB-YP374305YAK
CSB-EP374305YAK
CSB-BP374305YAK
CSB-MP374305YAK
CSB-EP374305YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosomal protein L11 methyltransferase (prmA) CSB-YP374306YAK
CSB-EP374306YAK
CSB-BP374306YAK
CSB-MP374306YAK
CSB-EP374306YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B tRNA (mo5U34)-methyltransferase CSB-YP374307YAK
CSB-EP374307YAK
CSB-BP374307YAK
CSB-MP374307YAK
CSB-EP374307YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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