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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neosartorya fischeri Probable ubiquitin carboxyl-terminal hydrolase creB (creB), partial CSB-YP373088NEZ
CSB-EP373088NEZ
CSB-BP373088NEZ
CSB-MP373088NEZ
CSB-EP373088NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Probable ubiquitination network signaling protein acrB (acrB), partial CSB-YP373089NEZ
CSB-EP373089NEZ
CSB-BP373089NEZ
CSB-MP373089NEZ
CSB-EP373089NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Glucose-repressible alcohol dehydrogenase transcriptional effector (ccr4), partial CSB-YP373090NEZ
CSB-EP373090NEZ
CSB-BP373090NEZ
CSB-MP373090NEZ
CSB-EP373090NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Cytosolic Fe-S cluster assembly factor NAR1 (NAR1), partial CSB-YP373091NEZ
CSB-EP373091NEZ
CSB-BP373091NEZ
CSB-MP373091NEZ
CSB-EP373091NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Plasma membrane fusion protein prm1 (prm1), partial CSB-YP373092NEZ1
CSB-EP373092NEZ1
CSB-BP373092NEZ1
CSB-MP373092NEZ1
CSB-EP373092NEZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Kynureninase 2 (bna5-2) CSB-YP373093NEZ
CSB-EP373093NEZ
CSB-BP373093NEZ
CSB-MP373093NEZ
CSB-EP373093NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Probable dipeptidyl peptidase 4 (dpp4), partial CSB-YP373094NEZ
CSB-EP373094NEZ
CSB-BP373094NEZ
CSB-MP373094NEZ
CSB-EP373094NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Pre-mRNA-splicing ATP-dependent RNA helicase prp28 (prp28), partial CSB-YP373095NEZ
CSB-EP373095NEZ
CSB-BP373095NEZ
CSB-MP373095NEZ
CSB-EP373095NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP373096NEZ1
CSB-EP373096NEZ1
CSB-BP373096NEZ1
CSB-MP373096NEZ1
CSB-EP373096NEZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Restriction of telomere capping protein 5 (rtc5), partial CSB-YP373097NEZ
CSB-EP373097NEZ
CSB-BP373097NEZ
CSB-MP373097NEZ
CSB-EP373097NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Formation of crista junctions protein 1 (FCJ1), partial CSB-YP373098NEZ1
CSB-EP373098NEZ1
CSB-BP373098NEZ1
CSB-MP373098NEZ1
CSB-EP373098NEZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Ribosome biogenesis protein ytm1 (ytm1) CSB-YP373099NEZ
CSB-EP373099NEZ
CSB-BP373099NEZ
CSB-MP373099NEZ
CSB-EP373099NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Tethering factor for nuclear proteasome sts1 (sts1) CSB-YP373100NEZ
CSB-EP373100NEZ
CSB-BP373100NEZ
CSB-MP373100NEZ
CSB-EP373100NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 5 (nut1), partial CSB-YP373101NEZ
CSB-EP373101NEZ
CSB-BP373101NEZ
CSB-MP373101NEZ
CSB-EP373101NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Probable pectin lyase A (pelA) CSB-YP373102NEZ
CSB-EP373102NEZ
CSB-BP373102NEZ
CSB-MP373102NEZ
CSB-EP373102NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri ATP-dependent RNA helicase dbp5 (dbp5) CSB-YP373103NEZ
CSB-EP373103NEZ
CSB-BP373103NEZ
CSB-MP373103NEZ
CSB-EP373103NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Methionine aminopeptidase 2 homolog NFIA_034070 (NFIA_034070) CSB-YP373104NEZ
CSB-EP373104NEZ
CSB-BP373104NEZ
CSB-MP373104NEZ
CSB-EP373104NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Sterol 3-beta-glucosyltransferase (atg26), partial CSB-YP373105NEZ
CSB-EP373105NEZ
CSB-BP373105NEZ
CSB-MP373105NEZ
CSB-EP373105NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Carbamoyl-phosphate synthase arginine-specific small chain (cpa1) CSB-YP373106NEZ
CSB-EP373106NEZ
CSB-BP373106NEZ
CSB-MP373106NEZ
CSB-EP373106NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri E3 ubiquitin ligase complex SCF subunit sconC (sconC) CSB-YP373107NEZ
CSB-EP373107NEZ
CSB-BP373107NEZ
CSB-MP373107NEZ
CSB-EP373107NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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