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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aspergillus clavatus ATP-dependent RNA helicase rok1 (rok1), partial CSB-YP373048AVC
CSB-EP373048AVC
CSB-BP373048AVC
CSB-MP373048AVC
CSB-EP373048AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Adenylosuccinate synthetase (ACLA_019890) CSB-YP373049AVC
CSB-EP373049AVC
CSB-BP373049AVC
CSB-MP373049AVC
CSB-EP373049AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable Xaa-Pro aminopeptidase ACLA_020440 (ACLA_020440) CSB-YP373050AVC
CSB-EP373050AVC
CSB-BP373050AVC
CSB-MP373050AVC
CSB-EP373050AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mitochondrial distribution and morphology protein 12 (mdm12) CSB-YP373051AVC
CSB-EP373051AVC
CSB-BP373051AVC
CSB-MP373051AVC
CSB-EP373051AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (mns1B) CSB-YP373052AVC
CSB-EP373052AVC
CSB-BP373052AVC
CSB-MP373052AVC
CSB-EP373052AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Histone H3 (hht1) CSB-YP373053AVC
CSB-EP373053AVC
CSB-BP373053AVC
CSB-MP373053AVC
CSB-EP373053AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Actin cytoskeleton-regulatory complex protein end3 (end3) CSB-YP373054AVC
CSB-EP373054AVC
CSB-BP373054AVC
CSB-MP373054AVC
CSB-EP373054AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Protein sey1 (sey1), partial CSB-YP373055AVC
CSB-EP373055AVC
CSB-BP373055AVC
CSB-MP373055AVC
CSB-EP373055AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Catabolic 3-dehydroquinase (qutE) CSB-YP373056AVC
CSB-EP373056AVC
CSB-BP373056AVC
CSB-MP373056AVC
CSB-EP373056AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Pre-mRNA-processing ATP-dependent RNA helicase prp5 (prp5), partial CSB-YP373057AVC
CSB-EP373057AVC
CSB-BP373057AVC
CSB-MP373057AVC
CSB-EP373057AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Nitrogen permease regulator 3 (npr3), partial CSB-YP373058AVC
CSB-EP373058AVC
CSB-BP373058AVC
CSB-MP373058AVC
CSB-EP373058AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus U3 small nucleolar RNA-associated protein 25 (utp25), partial CSB-YP373059AVC
CSB-EP373059AVC
CSB-BP373059AVC
CSB-MP373059AVC
CSB-EP373059AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable cytosolic iron-sulfur protein assembly protein 1 (cia1) CSB-YP373060AVC
CSB-EP373060AVC
CSB-BP373060AVC
CSB-MP373060AVC
CSB-EP373060AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Fe-S cluster assembly protein dre2 (dre2) CSB-YP373061AVC
CSB-EP373061AVC
CSB-BP373061AVC
CSB-MP373061AVC
CSB-EP373061AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Histone H2A.Z-specific chaperone chz1 (chz1) CSB-YP373062AVC
CSB-EP373062AVC
CSB-BP373062AVC
CSB-MP373062AVC
CSB-EP373062AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus ATP-dependent rRNA helicase rrp3 (rrp3) CSB-YP373063AVC
CSB-EP373063AVC
CSB-BP373063AVC
CSB-MP373063AVC
CSB-EP373063AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable beta-glucosidase A (bglA), partial CSB-YP373064AVC
CSB-EP373064AVC
CSB-BP373064AVC
CSB-MP373064AVC
CSB-EP373064AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus tRNA (guanine-N (7)-)-methyltransferase subunit trm82 CSB-YP373065AVC
CSB-EP373065AVC
CSB-BP373065AVC
CSB-MP373065AVC
CSB-EP373065AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Small COPII coat GTPase sar1 (sar1) CSB-YP373066AVC
CSB-EP373066AVC
CSB-BP373066AVC
CSB-MP373066AVC
CSB-EP373066AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable xyloglucan-specific endo-beta-1,4-glucanase A (xgeA) CSB-YP373067AVC
CSB-EP373067AVC
CSB-BP373067AVC
CSB-MP373067AVC
CSB-EP373067AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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