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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Azoarcus sp. Dihydroorotase (pyrC) CSB-YP374789AVL
CSB-EP374789AVL
CSB-BP374789AVL
CSB-MP374789AVL
CSB-EP374789AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP374790AVL
CSB-EP374790AVL
CSB-BP374790AVL
CSB-MP374790AVL
CSB-EP374790AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP374791AVL
CSB-EP374791AVL
CSB-BP374791AVL
CSB-MP374791AVL
CSB-EP374791AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Recombination protein RecR (recR) CSB-YP374792AVL
CSB-EP374792AVL
CSB-BP374792AVL
CSB-MP374792AVL
CSB-EP374792AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 60 kDa chaperonin 1 (groL1), partial CSB-YP374793AVL
CSB-EP374793AVL
CSB-BP374793AVL
CSB-MP374793AVL
CSB-EP374793AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP374794AVL
CSB-EP374794AVL
CSB-BP374794AVL
CSB-MP374794AVL
CSB-EP374794AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Chorismate synthase (aroC) CSB-YP374795AVL
CSB-EP374795AVL
CSB-BP374795AVL
CSB-MP374795AVL
CSB-EP374795AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Integration host factor subunit beta (ihfB) CSB-YP374796AVL
CSB-EP374796AVL
CSB-BP374796AVL
CSB-MP374796AVL
CSB-EP374796AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Integration host factor subunit alpha (ihfA) CSB-YP374797AVL
CSB-EP374797AVL
CSB-BP374797AVL
CSB-MP374797AVL
CSB-EP374797AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP374798AVL
CSB-EP374798AVL
CSB-BP374798AVL
CSB-MP374798AVL
CSB-EP374798AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UPF0758 protein azo1137 (azo1137) CSB-YP374799AVL
CSB-EP374799AVL
CSB-BP374799AVL
CSB-MP374799AVL
CSB-EP374799AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP374800AVL
CSB-EP374800AVL
CSB-BP374800AVL
CSB-MP374800AVL
CSB-EP374800AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP374801AVL
CSB-EP374801AVL
CSB-BP374801AVL
CSB-MP374801AVL
CSB-EP374801AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Isoleucine--tRNA ligase (ileS), partial CSB-YP374802AVL
CSB-EP374802AVL
CSB-BP374802AVL
CSB-MP374802AVL
CSB-EP374802AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Serine--tRNA ligase (serS) CSB-YP374803AVL
CSB-EP374803AVL
CSB-BP374803AVL
CSB-MP374803AVL
CSB-EP374803AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP374804AVL
CSB-EP374804AVL
CSB-BP374804AVL
CSB-MP374804AVL
CSB-EP374804AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Electron transport complex protein RnfB (rnfB) CSB-YP374805AVL
CSB-EP374805AVL
CSB-BP374805AVL
CSB-MP374805AVL
CSB-EP374805AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Glutamate--tRNA ligase (gltX) CSB-YP374806AVL
CSB-EP374806AVL
CSB-BP374806AVL
CSB-MP374806AVL
CSB-EP374806AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Lysine--tRNA ligase (lysS) CSB-YP374807AVL
CSB-EP374807AVL
CSB-BP374807AVL
CSB-MP374807AVL
CSB-EP374807AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. SsrA-binding protein (smpB) CSB-YP374808AVL
CSB-EP374808AVL
CSB-BP374808AVL
CSB-MP374808AVL
CSB-EP374808AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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