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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria meningitidis serogroup C / serotype 2a Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP374528NEX
CSB-EP374528NEX
CSB-BP374528NEX
CSB-MP374528NEX
CSB-EP374528NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP374529NEX
CSB-EP374529NEX
CSB-BP374529NEX
CSB-MP374529NEX
CSB-EP374529NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Membrane protein insertase YidC (yidC), partial CSB-YP374530NEX1
CSB-EP374530NEX1
CSB-BP374530NEX1
CSB-MP374530NEX1
CSB-EP374530NEX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 50S ribosomal protein L34 (rpmH) CSB-YP374531NEX
CSB-EP374531NEX
CSB-BP374531NEX
CSB-MP374531NEX
CSB-EP374531NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Tyrosine recombinase XerC (xerC) CSB-YP374532NEX
CSB-EP374532NEX
CSB-BP374532NEX
CSB-MP374532NEX
CSB-EP374532NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 3-dehydroquinate synthase (aroB) CSB-YP374533NEX
CSB-EP374533NEX
CSB-BP374533NEX
CSB-MP374533NEX
CSB-EP374533NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP374534NEX
CSB-EP374534NEX
CSB-BP374534NEX
CSB-MP374534NEX
CSB-EP374534NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Chaperone protein DnaK (dnaK), partial CSB-YP374535NEX
CSB-EP374535NEX
CSB-BP374535NEX
CSB-MP374535NEX
CSB-EP374535NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Putative iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP374536NEX
CSB-EP374536NEX
CSB-BP374536NEX
CSB-MP374536NEX
CSB-EP374536NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 50S ribosomal protein L19 (rplS) CSB-YP374537NEX
CSB-EP374537NEX
CSB-BP374537NEX
CSB-MP374537NEX
CSB-EP374537NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Outer-membrane lipoprotein carrier protein (lolA) CSB-YP374538NEX
CSB-EP374538NEX
CSB-BP374538NEX
CSB-MP374538NEX
CSB-EP374538NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP374539NEX
CSB-EP374539NEX
CSB-BP374539NEX
CSB-MP374539NEX
CSB-EP374539NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Dihydroorotase (pyrC) CSB-YP374540NEX
CSB-EP374540NEX
CSB-BP374540NEX
CSB-MP374540NEX
CSB-EP374540NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Tryptophan synthase beta chain (trpB) CSB-YP374541NEX
CSB-EP374541NEX
CSB-BP374541NEX
CSB-MP374541NEX
CSB-EP374541NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a 50S ribosomal protein L35 (rpmI) CSB-YP374542NEX
CSB-EP374542NEX
CSB-BP374542NEX
CSB-MP374542NEX
CSB-EP374542NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Integration host factor subunit alpha (ihfA) CSB-YP374543NEX
CSB-EP374543NEX
CSB-BP374543NEX
CSB-MP374543NEX
CSB-EP374543NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP374544NEX
CSB-EP374544NEX
CSB-BP374544NEX
CSB-MP374544NEX
CSB-EP374544NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Elongation factor 4 (lepA), partial CSB-YP374545NEX
CSB-EP374545NEX
CSB-BP374545NEX
CSB-MP374545NEX
CSB-EP374545NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a Peptidyl-tRNA hydrolase (pth) CSB-YP374546NEX
CSB-EP374546NEX
CSB-BP374546NEX
CSB-MP374546NEX
CSB-EP374546NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C / serotype 2a ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP374547NEX
CSB-EP374547NEX
CSB-BP374547NEX
CSB-MP374547NEX
CSB-EP374547NEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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