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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium bovis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP374468MOJ
CSB-EP374468MOJ
CSB-BP374468MOJ
CSB-MP374468MOJ
CSB-EP374468MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Cytidylate kinase (cmk) CSB-YP374469MOJ
CSB-EP374469MOJ
CSB-BP374469MOJ
CSB-MP374469MOJ
CSB-EP374469MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Putative succinate-semialdehyde dehydrogenase [NADP (+)] 2, partial CSB-YP374470MOJ
CSB-EP374470MOJ
CSB-BP374470MOJ
CSB-MP374470MOJ
CSB-EP374470MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Urease subunit beta (ureB) CSB-YP374471MOJ
CSB-EP374471MOJ
CSB-BP374471MOJ
CSB-MP374471MOJ
CSB-EP374471MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Antigen 85-B (fbpB) CSB-YP374472MOJ
CSB-BP374472MOJ
CSB-MP374472MOJ
CSB-EP374472MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Catalase-peroxidase (katG), partial CSB-YP374473MOJ
CSB-EP374473MOJ
CSB-BP374473MOJ
CSB-MP374473MOJ
CSB-EP374473MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Pup--protein ligase (pafA) CSB-YP374474MOJ
CSB-EP374474MOJ
CSB-BP374474MOJ
CSB-MP374474MOJ
CSB-EP374474MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Proteasome subunit beta (prcB) CSB-YP374475MOJ
CSB-EP374475MOJ
CSB-BP374475MOJ
CSB-MP374475MOJ
CSB-EP374475MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Proteasome-associated ATPase (mpa), partial CSB-YP374476MOJ
CSB-EP374476MOJ
CSB-BP374476MOJ
CSB-MP374476MOJ
CSB-EP374476MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Cell division protein SepF (sepF) CSB-YP374477MOJ
CSB-EP374477MOJ
CSB-BP374477MOJ
CSB-MP374477MOJ
CSB-EP374477MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Cobalamin synthase (cobS), partial CSB-YP374478MOJ1
CSB-EP374478MOJ1
CSB-BP374478MOJ1
CSB-MP374478MOJ1
CSB-EP374478MOJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Chaperone protein htpG (htpG), partial CSB-YP374479MOJ
CSB-EP374479MOJ
CSB-BP374479MOJ
CSB-MP374479MOJ
CSB-EP374479MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Ribonuclease Z (rnz) CSB-YP374480MOJ
CSB-EP374480MOJ
CSB-BP374480MOJ
CSB-MP374480MOJ
CSB-EP374480MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 30S ribosomal protein S20 (rpsT) CSB-YP374481MOJ
CSB-EP374481MOJ
CSB-BP374481MOJ
CSB-MP374481MOJ
CSB-EP374481MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP374482MOJ
CSB-EP374482MOJ
CSB-BP374482MOJ
CSB-MP374482MOJ
CSB-EP374482MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP374483MOJ
CSB-EP374483MOJ
CSB-BP374483MOJ
CSB-MP374483MOJ
CSB-EP374483MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Elongation factor P (efp) CSB-YP374484MOJ
CSB-EP374484MOJ
CSB-BP374484MOJ
CSB-MP374484MOJ
CSB-EP374484MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Putative Holliday junction resolvase (BCG_2577c) CSB-YP374485MOJ
CSB-EP374485MOJ
CSB-BP374485MOJ
CSB-MP374485MOJ
CSB-EP374485MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP374486MOJ
CSB-EP374486MOJ
CSB-BP374486MOJ
CSB-MP374486MOJ
CSB-EP374486MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis LexA repressor (lexA) CSB-YP374487MOJ
CSB-EP374487MOJ
CSB-BP374487MOJ
CSB-MP374487MOJ
CSB-EP374487MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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