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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella amazonensis 30S ribosomal protein S8 (rpsH) CSB-YP377669STK
CSB-EP377669STK
CSB-BP377669STK
CSB-MP377669STK
CSB-EP377669STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L36 (rpmJ) CSB-YP377670STK
CSB-EP377670STK
CSB-BP377670STK
CSB-MP377670STK
CSB-EP377670STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S4 (rpsD) CSB-YP377671STK
CSB-EP377671STK
CSB-BP377671STK
CSB-MP377671STK
CSB-EP377671STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP377672STK
CSB-EP377672STK
CSB-BP377672STK
CSB-MP377672STK
CSB-EP377672STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP377673STK
CSB-EP377673STK
CSB-BP377673STK
CSB-MP377673STK
CSB-EP377673STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP377674STK
CSB-EP377674STK
CSB-BP377674STK
CSB-MP377674STK
CSB-EP377674STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 2-isopropylmalate synthase (leuA), partial CSB-YP377675STK
CSB-EP377675STK
CSB-BP377675STK
CSB-MP377675STK
CSB-EP377675STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glycerol kinase (glpK) CSB-YP377676STK
CSB-EP377676STK
CSB-BP377676STK
CSB-MP377676STK
CSB-EP377676STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP377677STK
CSB-EP377677STK
CSB-BP377677STK
CSB-MP377677STK
CSB-EP377677STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Protein translocase subunit SecA (secA), partial CSB-YP377678STK
CSB-EP377678STK
CSB-BP377678STK
CSB-MP377678STK
CSB-EP377678STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP377679STK
CSB-EP377679STK
CSB-BP377679STK
CSB-MP377679STK
CSB-EP377679STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP377680STK
CSB-EP377680STK
CSB-BP377680STK
CSB-MP377680STK
CSB-EP377680STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP377681STK
CSB-EP377681STK
CSB-BP377681STK
CSB-MP377681STK
CSB-EP377681STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP377682STK
CSB-EP377682STK
CSB-BP377682STK
CSB-MP377682STK
CSB-EP377682STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP377683STK1
CSB-EP377683STK1
CSB-BP377683STK1
CSB-MP377683STK1
CSB-EP377683STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP377684STK
CSB-EP377684STK
CSB-BP377684STK
CSB-MP377684STK
CSB-EP377684STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S21 (rpsU) CSB-YP377685STK
CSB-EP377685STK
CSB-BP377685STK
CSB-MP377685STK
CSB-EP377685STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Undecaprenyl-diphosphatase (uppP), partial CSB-YP377686STK
CSB-EP377686STK
CSB-BP377686STK
CSB-MP377686STK
CSB-EP377686STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP377687STK
CSB-EP377687STK
CSB-BP377687STK
CSB-MP377687STK
CSB-EP377687STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis DNA mismatch repair protein mutH (mutH) CSB-YP377688STK
CSB-EP377688STK
CSB-BP377688STK
CSB-MP377688STK
CSB-EP377688STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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