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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium vanbaalenii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP377369MOQ
CSB-EP377369MOQ
CSB-BP377369MOQ
CSB-MP377369MOQ
CSB-EP377369MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Diaminopimelate epimerase (dapF) CSB-YP377370MOQ
CSB-EP377370MOQ
CSB-BP377370MOQ
CSB-MP377370MOQ
CSB-EP377370MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP377371MOQ
CSB-EP377371MOQ
CSB-BP377371MOQ
CSB-MP377371MOQ
CSB-EP377371MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP377372MOQ
CSB-EP377372MOQ
CSB-BP377372MOQ
CSB-MP377372MOQ
CSB-EP377372MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Threonine--tRNA ligase (thrS), partial CSB-YP377373MOQ
CSB-EP377373MOQ
CSB-BP377373MOQ
CSB-MP377373MOQ
CSB-EP377373MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP377374MOQ
CSB-EP377374MOQ
CSB-BP377374MOQ
CSB-MP377374MOQ
CSB-EP377374MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Histidine--tRNA ligase (hisS) CSB-YP377375MOQ
CSB-EP377375MOQ
CSB-BP377375MOQ
CSB-MP377375MOQ
CSB-EP377375MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Chorismate synthase (aroC) CSB-YP377376MOQ
CSB-EP377376MOQ
CSB-BP377376MOQ
CSB-MP377376MOQ
CSB-EP377376MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Elongation factor P (efp) CSB-YP377377MOQ
CSB-EP377377MOQ
CSB-BP377377MOQ
CSB-MP377377MOQ
CSB-EP377377MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP377378MOQ
CSB-EP377378MOQ
CSB-BP377378MOQ
CSB-MP377378MOQ
CSB-EP377378MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii S-adenosylmethionine synthase (metK) CSB-YP377379MOQ
CSB-EP377379MOQ
CSB-BP377379MOQ
CSB-MP377379MOQ
CSB-EP377379MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP377380MOQ
CSB-EP377380MOQ
CSB-BP377380MOQ
CSB-MP377380MOQ
CSB-EP377380MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii UvrABC system protein C (uvrC), partial CSB-YP377381MOQ
CSB-EP377381MOQ
CSB-BP377381MOQ
CSB-MP377381MOQ
CSB-EP377381MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Putative sporulation transcription regulator WhiA (whiA) CSB-YP377382MOQ
CSB-EP377382MOQ
CSB-BP377382MOQ
CSB-MP377382MOQ
CSB-EP377382MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP377383MOQ
CSB-EP377383MOQ
CSB-BP377383MOQ
CSB-MP377383MOQ
CSB-EP377383MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Biotin synthase (bioB) CSB-YP377384MOQ
CSB-EP377384MOQ
CSB-BP377384MOQ
CSB-MP377384MOQ
CSB-EP377384MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP377385MOQ
CSB-EP377385MOQ
CSB-BP377385MOQ
CSB-MP377385MOQ
CSB-EP377385MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP377386MOQ
CSB-EP377386MOQ
CSB-BP377386MOQ
CSB-MP377386MOQ
CSB-EP377386MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D (SSC5D), partial CSB-YP377387HU
CSB-EP377387HU
CSB-BP377387HU
CSB-MP377387HU
CSB-EP377387HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein FLJ41423 CSB-YP377388HU
CSB-EP377388HU
CSB-BP377388HU
CSB-MP377388HU
CSB-EP377388HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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