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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidovorax citrulli 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP378550AUB
CSB-EP378550AUB
CSB-BP378550AUB
CSB-MP378550AUB
CSB-EP378550AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Chaperone protein DnaJ (dnaJ) CSB-YP378551AUB
CSB-EP378551AUB
CSB-BP378551AUB
CSB-MP378551AUB
CSB-EP378551AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli 30S ribosomal protein S6 (rpsF) CSB-YP378552AUB
CSB-EP378552AUB
CSB-BP378552AUB
CSB-MP378552AUB
CSB-EP378552AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli 30S ribosomal protein S15 (rpsO) CSB-YP378553AUB
CSB-EP378553AUB
CSB-BP378553AUB
CSB-MP378553AUB
CSB-EP378553AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP378554AUB
CSB-EP378554AUB
CSB-BP378554AUB
CSB-MP378554AUB
CSB-EP378554AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Putative phosphotransferase Aave_1381 (Aave_1381) CSB-YP378555AUB
CSB-EP378555AUB
CSB-BP378555AUB
CSB-MP378555AUB
CSB-EP378555AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli GTPase Der (der) CSB-YP378556AUB
CSB-EP378556AUB
CSB-BP378556AUB
CSB-MP378556AUB
CSB-EP378556AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Trigger factor (tig) CSB-YP378557AUB
CSB-EP378557AUB
CSB-BP378557AUB
CSB-MP378557AUB
CSB-EP378557AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP378558AUB1
CSB-EP378558AUB1
CSB-BP378558AUB1
CSB-MP378558AUB1
CSB-EP378558AUB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Elongation factor P (efp) CSB-YP378559AUB
CSB-EP378559AUB
CSB-BP378559AUB
CSB-MP378559AUB
CSB-EP378559AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Uridylate kinase (pyrH) CSB-YP378560AUB
CSB-EP378560AUB
CSB-BP378560AUB
CSB-MP378560AUB
CSB-EP378560AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP378561AUB
CSB-EP378561AUB
CSB-BP378561AUB
CSB-MP378561AUB
CSB-EP378561AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli 30S ribosomal protein S16 (rpsP) CSB-YP378562AUB
CSB-EP378562AUB
CSB-BP378562AUB
CSB-MP378562AUB
CSB-EP378562AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli tRNA (guanine-N (1)-)-methyltransferase CSB-YP378563AUB
CSB-EP378563AUB
CSB-BP378563AUB
CSB-MP378563AUB
CSB-EP378563AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP378564AUB
CSB-EP378564AUB
CSB-BP378564AUB
CSB-MP378564AUB
CSB-EP378564AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP378565AUB
CSB-EP378565AUB
CSB-BP378565AUB
CSB-MP378565AUB
CSB-EP378565AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Cysteine--tRNA ligase (cysS) CSB-YP378566AUB
CSB-EP378566AUB
CSB-BP378566AUB
CSB-MP378566AUB
CSB-EP378566AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP378567AUB
CSB-EP378567AUB
CSB-BP378567AUB
CSB-MP378567AUB
CSB-EP378567AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Disulfide bond formation protein B (dsbB), partial CSB-YP378568AUB1
CSB-EP378568AUB1
CSB-BP378568AUB1
CSB-MP378568AUB1
CSB-EP378568AUB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli UPF0434 protein Aave_2563 (Aave_2563) CSB-YP378569AUB
CSB-EP378569AUB
CSB-BP378569AUB
CSB-MP378569AUB
CSB-EP378569AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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