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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Marinobacter aquaeolei DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP378630MNI
CSB-EP378630MNI
CSB-BP378630MNI
CSB-MP378630MNI
CSB-EP378630MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP378631MNI
CSB-EP378631MNI
CSB-BP378631MNI
CSB-MP378631MNI
CSB-EP378631MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ATP-dependent protease subunit HslV (hslV) CSB-YP378632MNI
CSB-EP378632MNI
CSB-BP378632MNI
CSB-MP378632MNI
CSB-EP378632MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 50S ribosomal protein L31 (rpmE) CSB-YP378633MNI
CSB-EP378633MNI
CSB-BP378633MNI
CSB-MP378633MNI
CSB-EP378633MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Transcriptional repressor NrdR (nrdR) CSB-YP378634MNI
CSB-EP378634MNI
CSB-BP378634MNI
CSB-MP378634MNI
CSB-EP378634MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 50S ribosomal protein L21 (rplU) CSB-YP378635MNI
CSB-EP378635MNI
CSB-BP378635MNI
CSB-MP378635MNI
CSB-EP378635MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 30S ribosomal protein S20 (rpsT) CSB-YP378636MNI
CSB-EP378636MNI
CSB-BP378636MNI
CSB-MP378636MNI
CSB-EP378636MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Ketol-acid reductoisomerase (ilvC) CSB-YP378637MNI
CSB-EP378637MNI
CSB-BP378637MNI
CSB-MP378637MNI
CSB-EP378637MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP378638MNI
CSB-EP378638MNI
CSB-BP378638MNI
CSB-MP378638MNI
CSB-EP378638MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP378639MNI
CSB-EP378639MNI
CSB-BP378639MNI
CSB-MP378639MNI
CSB-EP378639MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP378640MNI
CSB-EP378640MNI
CSB-BP378640MNI
CSB-MP378640MNI
CSB-EP378640MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Protein-L-isoaspartate O-methyltransferase 1 (pcm1) CSB-YP378641MNI
CSB-EP378641MNI
CSB-BP378641MNI
CSB-MP378641MNI
CSB-EP378641MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Electron transport complex protein RnfA (rnfA), partial CSB-YP378642MNI1
CSB-EP378642MNI1
CSB-BP378642MNI1
CSB-MP378642MNI1
CSB-EP378642MNI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Putative pterin-4-alpha-carbinolamine dehydratase (Maqu_0976) CSB-YP378643MNI
CSB-EP378643MNI
CSB-BP378643MNI
CSB-MP378643MNI
CSB-EP378643MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP378644MNI1
CSB-EP378644MNI1
CSB-BP378644MNI1
CSB-MP378644MNI1
CSB-EP378644MNI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Catalase-peroxidase (katG), partial CSB-YP378645MNI
CSB-EP378645MNI
CSB-BP378645MNI
CSB-MP378645MNI
CSB-EP378645MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Flagellar P-ring protein (flgI) CSB-YP378646MNI
CSB-EP378646MNI
CSB-BP378646MNI
CSB-MP378646MNI
CSB-EP378646MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP378647MNI
CSB-EP378647MNI
CSB-BP378647MNI
CSB-MP378647MNI
CSB-EP378647MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP378648MNI
CSB-EP378648MNI
CSB-BP378648MNI
CSB-MP378648MNI
CSB-EP378648MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Glycogen synthase (glgA) CSB-YP378649MNI
CSB-EP378649MNI
CSB-BP378649MNI
CSB-MP378649MNI
CSB-EP378649MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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