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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Marinobacter aquaeolei Ribonuclease H (rnhA) CSB-YP378650MNI
CSB-EP378650MNI
CSB-BP378650MNI
CSB-MP378650MNI
CSB-EP378650MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP378651MNI
CSB-EP378651MNI
CSB-BP378651MNI
CSB-MP378651MNI
CSB-EP378651MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP378652MNI
CSB-EP378652MNI
CSB-BP378652MNI
CSB-MP378652MNI
CSB-EP378652MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei UPF0745 protein Maqu_1609 (Maqu_1609) CSB-YP378653MNI
CSB-EP378653MNI
CSB-BP378653MNI
CSB-MP378653MNI
CSB-EP378653MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei UPF0133 protein Maqu_1612 (Maqu_1612) CSB-YP378654MNI
CSB-EP378654MNI
CSB-BP378654MNI
CSB-MP378654MNI
CSB-EP378654MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Quinolinate synthase A (nadA) CSB-YP378655MNI
CSB-EP378655MNI
CSB-BP378655MNI
CSB-MP378655MNI
CSB-EP378655MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP378656MNI
CSB-EP378656MNI
CSB-BP378656MNI
CSB-MP378656MNI
CSB-EP378656MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Aspartate--tRNA ligase (aspS), partial CSB-YP378657MNI
CSB-EP378657MNI
CSB-BP378657MNI
CSB-MP378657MNI
CSB-EP378657MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP378658MNI
CSB-EP378658MNI
CSB-BP378658MNI
CSB-MP378658MNI
CSB-EP378658MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP378659MNI
CSB-EP378659MNI
CSB-BP378659MNI
CSB-MP378659MNI
CSB-EP378659MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP378660MNI
CSB-EP378660MNI
CSB-BP378660MNI
CSB-MP378660MNI
CSB-EP378660MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP378661MNI
CSB-EP378661MNI
CSB-BP378661MNI
CSB-MP378661MNI
CSB-EP378661MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP378662MNI
CSB-EP378662MNI
CSB-BP378662MNI
CSB-MP378662MNI
CSB-EP378662MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 50S ribosomal protein L32 (rpmF) CSB-YP378663MNI
CSB-EP378663MNI
CSB-BP378663MNI
CSB-MP378663MNI
CSB-EP378663MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Putative phosphotransferase Maqu_1884 (Maqu_1884) CSB-YP378664MNI
CSB-EP378664MNI
CSB-BP378664MNI
CSB-MP378664MNI
CSB-EP378664MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP378665MNI
CSB-EP378665MNI
CSB-BP378665MNI
CSB-MP378665MNI
CSB-EP378665MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Uracil phosphoribosyltransferase (upp) CSB-YP378666MNI
CSB-EP378666MNI
CSB-BP378666MNI
CSB-MP378666MNI
CSB-EP378666MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 60 kDa chaperonin (groL), partial CSB-YP378667MNI
CSB-EP378667MNI
CSB-BP378667MNI
CSB-MP378667MNI
CSB-EP378667MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 10 kDa chaperonin (groS) CSB-YP378668MNI
CSB-EP378668MNI
CSB-BP378668MNI
CSB-MP378668MNI
CSB-EP378668MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 50S ribosomal protein L35 (rpmI) CSB-YP378669MNI
CSB-EP378669MNI
CSB-BP378669MNI
CSB-MP378669MNI
CSB-EP378669MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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