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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Verminephrobacter eiseniae UvrABC system protein C (uvrC), partial CSB-YP380450VDX
CSB-EP380450VDX
CSB-BP380450VDX
CSB-MP380450VDX
CSB-EP380450VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Ribosome-binding factor A (rbfA) CSB-YP380451VDX
CSB-EP380451VDX
CSB-BP380451VDX
CSB-MP380451VDX
CSB-EP380451VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Acetaldehyde dehydrogenase (Veis_2782) CSB-YP380452VDX
CSB-EP380452VDX
CSB-BP380452VDX
CSB-MP380452VDX
CSB-EP380452VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP380453VDX
CSB-EP380453VDX
CSB-BP380453VDX
CSB-MP380453VDX
CSB-EP380453VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 30S ribosomal protein S15 (rpsO) CSB-YP380454VDX
CSB-EP380454VDX
CSB-BP380454VDX
CSB-MP380454VDX
CSB-EP380454VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Threonine--tRNA ligase (thrS), partial CSB-YP380455VDX
CSB-EP380455VDX
CSB-BP380455VDX
CSB-MP380455VDX
CSB-EP380455VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP380456VDX
CSB-EP380456VDX
CSB-BP380456VDX
CSB-MP380456VDX
CSB-EP380456VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP380457VDX1
CSB-EP380457VDX1
CSB-BP380457VDX1
CSB-MP380457VDX1
CSB-EP380457VDX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP380458VDX
CSB-EP380458VDX
CSB-BP380458VDX
CSB-MP380458VDX
CSB-EP380458VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Glutamate--tRNA ligase (gltX) CSB-YP380459VDX
CSB-EP380459VDX
CSB-BP380459VDX
CSB-MP380459VDX
CSB-EP380459VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae UPF0234 protein Veis_3464 (Veis_3464) CSB-YP380460VDX
CSB-EP380460VDX
CSB-BP380460VDX
CSB-MP380460VDX
CSB-EP380460VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Serine--tRNA ligase (serS) CSB-YP380461VDX
CSB-EP380461VDX
CSB-BP380461VDX
CSB-MP380461VDX
CSB-EP380461VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae UPF0761 membrane protein Veis_3782 (Veis_3782), partial CSB-YP380462VDX1
CSB-EP380462VDX1
CSB-BP380462VDX1
CSB-MP380462VDX1
CSB-EP380462VDX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Ribosome maturation factor RimM (rimM) CSB-YP380463VDX
CSB-EP380463VDX
CSB-BP380463VDX
CSB-MP380463VDX
CSB-EP380463VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Ferredoxin--NADP reductase (Veis_4316) CSB-YP380464VDX
CSB-EP380464VDX
CSB-BP380464VDX
CSB-MP380464VDX
CSB-EP380464VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP380465VDX
CSB-EP380465VDX
CSB-BP380465VDX
CSB-MP380465VDX
CSB-EP380465VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP380466VDX
CSB-EP380466VDX
CSB-BP380466VDX
CSB-MP380466VDX
CSB-EP380466VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP380467VDX
CSB-EP380467VDX
CSB-BP380467VDX
CSB-MP380467VDX
CSB-EP380467VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae ATP-dependent protease subunit HslV (hslV) CSB-YP380468VDX
CSB-EP380468VDX
CSB-BP380468VDX
CSB-MP380468VDX
CSB-EP380468VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP380469VDX
CSB-EP380469VDX
CSB-BP380469VDX
CSB-MP380469VDX
CSB-EP380469VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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