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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidovorax sp. UPF0301 protein Ajs_3573 (Ajs_3573) CSB-YP380950AUC
CSB-EP380950AUC
CSB-BP380950AUC
CSB-MP380950AUC
CSB-EP380950AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP380951AUC
CSB-EP380951AUC
CSB-BP380951AUC
CSB-MP380951AUC
CSB-EP380951AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Protein MraZ (mraZ) CSB-YP380952AUC
CSB-EP380952AUC
CSB-BP380952AUC
CSB-MP380952AUC
CSB-EP380952AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP380953AUC
CSB-EP380953AUC
CSB-BP380953AUC
CSB-MP380953AUC
CSB-EP380953AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Protease HtpX homolog (htpX), partial CSB-YP380954AUC1
CSB-EP380954AUC1
CSB-BP380954AUC1
CSB-MP380954AUC1
CSB-EP380954AUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP380955AUC
CSB-EP380955AUC
CSB-BP380955AUC
CSB-MP380955AUC
CSB-EP380955AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 50S ribosomal protein L11 (rplK) CSB-YP380956AUC
CSB-EP380956AUC
CSB-BP380956AUC
CSB-MP380956AUC
CSB-EP380956AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Leucine--tRNA ligase (leuS), partial CSB-YP380957AUC
CSB-EP380957AUC
CSB-BP380957AUC
CSB-MP380957AUC
CSB-EP380957AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP380958AUC
CSB-EP380958AUC
CSB-BP380958AUC
CSB-MP380958AUC
CSB-EP380958AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. tRNA modification GTPase MnmE (mnmE) CSB-YP380959AUC
CSB-EP380959AUC
CSB-BP380959AUC
CSB-MP380959AUC
CSB-EP380959AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP380960VDX
CSB-EP380960VDX
CSB-BP380960VDX
CSB-MP380960VDX
CSB-EP380960VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP380961VDX
CSB-EP380961VDX
CSB-BP380961VDX
CSB-MP380961VDX
CSB-EP380961VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Octanoyltransferase (lipB) CSB-YP380962VDX
CSB-EP380962VDX
CSB-BP380962VDX
CSB-MP380962VDX
CSB-EP380962VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Recombination protein RecR (recR) CSB-YP380963VDX
CSB-EP380963VDX
CSB-BP380963VDX
CSB-MP380963VDX
CSB-EP380963VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae L-rhamnonate dehydratase (rhmD) CSB-YP380964VDX
CSB-EP380964VDX
CSB-BP380964VDX
CSB-MP380964VDX
CSB-EP380964VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP380965VDX
CSB-EP380965VDX
CSB-BP380965VDX
CSB-MP380965VDX
CSB-EP380965VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L25 (rplY) CSB-YP380966VDX
CSB-EP380966VDX
CSB-BP380966VDX
CSB-MP380966VDX
CSB-EP380966VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Gamma-glutamyl phosphate reductase (proA) CSB-YP380967VDX
CSB-EP380967VDX
CSB-BP380967VDX
CSB-MP380967VDX
CSB-EP380967VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP380968VDX
CSB-EP380968VDX
CSB-BP380968VDX
CSB-MP380968VDX
CSB-EP380968VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Leucine--tRNA ligase (leuS), partial CSB-YP380969VDX
CSB-EP380969VDX
CSB-BP380969VDX
CSB-MP380969VDX
CSB-EP380969VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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