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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfovibrio vulgaris subsp. vulgaris Cobalamin synthase (cobS), partial CSB-YP380210DHY1
CSB-EP380210DHY1
CSB-BP380210DHY1
CSB-MP380210DHY1
CSB-EP380210DHY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Octanoyltransferase (lipB) CSB-YP380211DHY
CSB-EP380211DHY
CSB-BP380211DHY
CSB-MP380211DHY
CSB-EP380211DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Chorismate synthase (aroC) CSB-YP380212DHY
CSB-EP380212DHY
CSB-BP380212DHY
CSB-MP380212DHY
CSB-EP380212DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 30S ribosomal protein S16 (rpsP) CSB-YP380213DHY
CSB-EP380213DHY
CSB-BP380213DHY
CSB-MP380213DHY
CSB-EP380213DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Ribonuclease HII (rnhB) CSB-YP380214DHY
CSB-EP380214DHY
CSB-BP380214DHY
CSB-MP380214DHY
CSB-EP380214DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris UPF0102 protein Dvul_2148 (Dvul_2148) CSB-YP380215DHY
CSB-EP380215DHY
CSB-BP380215DHY
CSB-MP380215DHY
CSB-EP380215DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP380216DHY
CSB-EP380216DHY
CSB-BP380216DHY
CSB-MP380216DHY
CSB-EP380216DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Flagellar L-ring protein (flgH) CSB-YP380217DHY
CSB-EP380217DHY
CSB-BP380217DHY
CSB-MP380217DHY
CSB-EP380217DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP380218DHY
CSB-EP380218DHY
CSB-BP380218DHY
CSB-MP380218DHY
CSB-EP380218DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris UPF0597 protein Dvul_2496 (Dvul_2496) CSB-YP380219DHY
CSB-EP380219DHY
CSB-BP380219DHY
CSB-MP380219DHY
CSB-EP380219DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP380220DHY
CSB-EP380220DHY
CSB-BP380220DHY
CSB-MP380220DHY
CSB-EP380220DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Serine--tRNA ligase (serS) CSB-YP380221DHY
CSB-EP380221DHY
CSB-BP380221DHY
CSB-MP380221DHY
CSB-EP380221DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP380222DHY
CSB-EP380222DHY
CSB-BP380222DHY
CSB-MP380222DHY
CSB-EP380222DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP380223DHY1
CSB-EP380223DHY1
CSB-BP380223DHY1
CSB-MP380223DHY1
CSB-EP380223DHY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP380224PYT
CSB-EP380224PYT
CSB-BP380224PYT
CSB-MP380224PYT
CSB-EP380224PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Orotate phosphoribosyltransferase (pyrE) CSB-YP380225PYT
CSB-EP380225PYT
CSB-BP380225PYT
CSB-MP380225PYT
CSB-EP380225PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 30S ribosomal protein S7 (rpsG) CSB-YP380226PYT
CSB-EP380226PYT
CSB-BP380226PYT
CSB-MP380226PYT
CSB-EP380226PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L4 (rplD) CSB-YP380227PYT
CSB-EP380227PYT
CSB-BP380227PYT
CSB-MP380227PYT
CSB-EP380227PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L23 (rplW) CSB-YP380228PYT
CSB-EP380228PYT
CSB-BP380228PYT
CSB-MP380228PYT
CSB-EP380228PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L29 (rpmC) CSB-YP380229PYT
CSB-EP380229PYT
CSB-BP380229PYT
CSB-MP380229PYT
CSB-EP380229PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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