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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidovorax sp. Probable rRNA maturation factor (Ajs_3702) CSB-YP380410AUC
CSB-EP380410AUC
CSB-BP380410AUC
CSB-MP380410AUC
CSB-EP380410AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Dihydroorotase (pyrC) CSB-YP380411AUC
CSB-EP380411AUC
CSB-BP380411AUC
CSB-MP380411AUC
CSB-EP380411AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 50S ribosomal protein L7/L12 (rplL) CSB-YP380412AUC
CSB-EP380412AUC
CSB-BP380412AUC
CSB-MP380412AUC
CSB-EP380412AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Elongation factor Tu 2 (tuf2) CSB-YP380413AUC
CSB-EP380413AUC
CSB-BP380413AUC
CSB-MP380413AUC
CSB-EP380413AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. HPr kinase/phosphorylase (hprK) CSB-YP380414AUC
CSB-EP380414AUC
CSB-BP380414AUC
CSB-MP380414AUC
CSB-EP380414AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP380415AUC
CSB-EP380415AUC
CSB-BP380415AUC
CSB-MP380415AUC
CSB-EP380415AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Acylphosphatase (acyP) CSB-YP380416AUC
CSB-EP380416AUC
CSB-BP380416AUC
CSB-MP380416AUC
CSB-EP380416AUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP380417VDX
CSB-EP380417VDX
CSB-BP380417VDX
CSB-MP380417VDX
CSB-EP380417VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP380418VDX
CSB-EP380418VDX
CSB-BP380418VDX
CSB-MP380418VDX
CSB-EP380418VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 5'-nucleotidase surE (surE) CSB-YP380419VDX
CSB-EP380419VDX
CSB-BP380419VDX
CSB-MP380419VDX
CSB-EP380419VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP380420VDX
CSB-EP380420VDX
CSB-BP380420VDX
CSB-MP380420VDX
CSB-EP380420VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Ribonuclease H (rnhA) CSB-YP380421VDX
CSB-EP380421VDX
CSB-BP380421VDX
CSB-MP380421VDX
CSB-EP380421VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae UPF0102 protein Veis_0630 (Veis_0630) CSB-YP380422VDX
CSB-EP380422VDX
CSB-BP380422VDX
CSB-MP380422VDX
CSB-EP380422VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L19 (rplS) CSB-YP380423VDX
CSB-EP380423VDX
CSB-BP380423VDX
CSB-MP380423VDX
CSB-EP380423VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Protein grpE (grpE) CSB-YP380424VDX
CSB-EP380424VDX
CSB-BP380424VDX
CSB-MP380424VDX
CSB-EP380424VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L9 (rplI) CSB-YP380425VDX
CSB-EP380425VDX
CSB-BP380425VDX
CSB-MP380425VDX
CSB-EP380425VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP380426VDX
CSB-EP380426VDX
CSB-BP380426VDX
CSB-MP380426VDX
CSB-EP380426VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP380427VDX
CSB-EP380427VDX
CSB-BP380427VDX
CSB-MP380427VDX
CSB-EP380427VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Transcription elongation factor GreA (greA) CSB-YP380428VDX
CSB-EP380428VDX
CSB-BP380428VDX
CSB-MP380428VDX
CSB-EP380428VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Protease HtpX homolog (htpX), partial CSB-YP380429VDX1
CSB-EP380429VDX1
CSB-BP380429VDX1
CSB-MP380429VDX1
CSB-EP380429VDX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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