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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aspergillus niger Putative metallocarboxypeptidase ecm14 (ecm14), partial CSB-YP382155AVE
CSB-EP382155AVE
CSB-BP382155AVE
CSB-MP382155AVE
CSB-EP382155AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable exopolygalacturonase X (pgaX) CSB-YP382156AVE
CSB-EP382156AVE
CSB-BP382156AVE
CSB-MP382156AVE
CSB-EP382156AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Assembly factor cbp4 (cbp4), partial CSB-YP382157AVE
CSB-EP382157AVE
CSB-BP382157AVE
CSB-MP382157AVE
CSB-EP382157AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Topoisomerase 1-associated factor 1 (tof1), partial CSB-YP382158AVE
CSB-EP382158AVE
CSB-BP382158AVE
CSB-MP382158AVE
CSB-EP382158AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable rhamnogalacturonate lyase A (rglA), partial CSB-YP382159AVE
CSB-EP382159AVE
CSB-BP382159AVE
CSB-MP382159AVE
CSB-EP382159AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable cutinase 1 (An14g02170) CSB-YP382160AVE
CSB-EP382160AVE
CSB-BP382160AVE
CSB-MP382160AVE
CSB-EP382160AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Lysophospholipase nte1 (nte1), partial CSB-YP382161AVE
CSB-EP382161AVE
CSB-BP382161AVE
CSB-MP382161AVE
CSB-EP382161AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Adenylyltransferase and sulfurtransferase uba4 (uba4) CSB-YP382162AVE
CSB-EP382162AVE
CSB-BP382162AVE
CSB-MP382162AVE
CSB-EP382162AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable alpha-glucuronidase A (aguA), partial CSB-YP382163AVE
CSB-EP382163AVE
CSB-BP382163AVE
CSB-MP382163AVE
CSB-EP382163AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger 60S ribosomal subunit assembly/export protein loc1 (loc1) CSB-YP382164AVE
CSB-EP382164AVE
CSB-BP382164AVE
CSB-MP382164AVE
CSB-EP382164AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Cell division cycle protein 123 (cdc123) CSB-YP382165AVE
CSB-EP382165AVE
CSB-BP382165AVE
CSB-MP382165AVE
CSB-EP382165AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable alpha-N-arabinofuranosidase B (abfB) CSB-YP382166AVE
CSB-EP382166AVE
CSB-BP382166AVE
CSB-MP382166AVE
CSB-EP382166AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable endopolygalacturonase II (pgaII) CSB-YP382167AVE
CSB-EP382167AVE
CSB-BP382167AVE
CSB-MP382167AVE
CSB-EP382167AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Cyanate hydratase (cyn1) CSB-YP382168AVE
CSB-EP382168AVE
CSB-BP382168AVE
CSB-MP382168AVE
CSB-EP382168AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable pectin lyase F (pelF) CSB-YP382169AVE
CSB-EP382169AVE
CSB-BP382169AVE
CSB-MP382169AVE
CSB-EP382169AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Ribosome-releasing factor 2, mitochondrial (mef2), partial CSB-YP382170AVE
CSB-EP382170AVE
CSB-BP382170AVE
CSB-MP382170AVE
CSB-EP382170AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Putative DNA helicase ino80 (ino80), partial CSB-YP382171AVE
CSB-EP382171AVE
CSB-BP382171AVE
CSB-MP382171AVE
CSB-EP382171AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase dbp7 (dbp7), partial CSB-YP382172AVE
CSB-EP382172AVE
CSB-BP382172AVE
CSB-MP382172AVE
CSB-EP382172AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger MYND-type zinc finger protein samB (samB), partial CSB-YP382173AVE
CSB-EP382173AVE
CSB-BP382173AVE
CSB-MP382173AVE
CSB-EP382173AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Dol-P-Man:Man (5)GlcNAc (2)-PP-Dol alpha-1,3-mannosyltransferase, partial CSB-YP382174AVE
CSB-EP382174AVE
CSB-BP382174AVE
CSB-MP382174AVE
CSB-EP382174AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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