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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP382054PZF
CSB-EP382054PZF
CSB-BP382054PZF
CSB-MP382054PZF
CSB-EP382054PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Gamma-glutamyl phosphate reductase (proA) CSB-YP382055PZF
CSB-EP382055PZF
CSB-BP382055PZF
CSB-MP382055PZF
CSB-EP382055PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP382056PZF
CSB-EP382056PZF
CSB-BP382056PZF
CSB-MP382056PZF
CSB-EP382056PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP382057PZF
CSB-EP382057PZF
CSB-BP382057PZF
CSB-MP382057PZF
CSB-EP382057PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP382058PZF
CSB-EP382058PZF
CSB-BP382058PZF
CSB-MP382058PZF
CSB-EP382058PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP382059PZF
CSB-EP382059PZF
CSB-BP382059PZF
CSB-MP382059PZF
CSB-EP382059PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein M (psbM), partial CSB-YP382060PZF
CSB-EP382060PZF
CSB-BP382060PZF
CSB-MP382060PZF
CSB-EP382060PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine synthase (metK) CSB-YP382061PZF
CSB-EP382061PZF
CSB-BP382061PZF
CSB-MP382061PZF
CSB-EP382061PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L3 (rplC) CSB-YP382062PZF
CSB-EP382062PZF
CSB-BP382062PZF
CSB-MP382062PZF
CSB-EP382062PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L16 (rplP) CSB-YP382063PZF
CSB-EP382063PZF
CSB-BP382063PZF
CSB-MP382063PZF
CSB-EP382063PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L14 (rplN) CSB-YP382064PZF
CSB-EP382064PZF
CSB-BP382064PZF
CSB-MP382064PZF
CSB-EP382064PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L5 (rplE) CSB-YP382065PZF
CSB-EP382065PZF
CSB-BP382065PZF
CSB-MP382065PZF
CSB-EP382065PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L36 (rpmJ) CSB-YP382066PZF
CSB-EP382066PZF
CSB-BP382066PZF
CSB-MP382066PZF
CSB-EP382066PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP382067PZF
CSB-EP382067PZF
CSB-BP382067PZF
CSB-MP382067PZF
CSB-EP382067PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L13 (rplM) CSB-YP382068PZF
CSB-EP382068PZF
CSB-BP382068PZF
CSB-MP382068PZF
CSB-EP382068PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP382069PZF
CSB-EP382069PZF
CSB-BP382069PZF
CSB-MP382069PZF
CSB-EP382069PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chorismate synthase (aroC) CSB-YP382070PZF
CSB-EP382070PZF
CSB-BP382070PZF
CSB-MP382070PZF
CSB-EP382070PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA (guanine-N (7)-)-methyltransferase CSB-YP382071PZF
CSB-EP382071PZF
CSB-BP382071PZF
CSB-MP382071PZF
CSB-EP382071PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP382072PZF
CSB-EP382072PZF
CSB-BP382072PZF
CSB-MP382072PZF
CSB-EP382072PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cobalamin synthase (cobS), partial CSB-YP382073PZF
CSB-EP382073PZF
CSB-BP382073PZF
CSB-MP382073PZF
CSB-EP382073PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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