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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M5 Glutamate racemase (murI) CSB-YP382255SUQ
CSB-EP382255SUQ
CSB-BP382255SUQ
CSB-MP382255SUQ
CSB-EP382255SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP382256SUQ
CSB-EP382256SUQ
CSB-BP382256SUQ
CSB-MP382256SUQ
CSB-EP382256SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Protease HtpX homolog (htpX), partial CSB-YP382257SUQ1
CSB-EP382257SUQ1
CSB-BP382257SUQ1
CSB-MP382257SUQ1
CSB-EP382257SUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Probable transcriptional regulatory protein SpyM51586 (SpyM51586) CSB-YP382258SUQ
CSB-EP382258SUQ
CSB-BP382258SUQ
CSB-MP382258SUQ
CSB-EP382258SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP382259SUQ
CSB-EP382259SUQ
CSB-BP382259SUQ
CSB-MP382259SUQ
CSB-EP382259SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 DNA polymerase III polC-type (polC), partial CSB-YP382260SUQ
CSB-EP382260SUQ
CSB-BP382260SUQ
CSB-MP382260SUQ
CSB-EP382260SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Ribosomal protein L11 methyltransferase (prmA) CSB-YP382261SUQ
CSB-EP382261SUQ
CSB-BP382261SUQ
CSB-MP382261SUQ
CSB-EP382261SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 60 kDa chaperonin (groL), partial CSB-YP382262SUQ
CSB-EP382262SUQ
CSB-BP382262SUQ
CSB-MP382262SUQ
CSB-EP382262SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 10 kDa chaperonin (groS) CSB-YP382263SUQ
CSB-EP382263SUQ
CSB-BP382263SUQ
CSB-MP382263SUQ
CSB-EP382263SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UPF0297 protein SpyM51756 (SpyM51756) CSB-YP382264SUQ
CSB-EP382264SUQ
CSB-BP382264SUQ
CSB-MP382264SUQ
CSB-EP382264SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Putative competence-damage inducible protein (cinA) CSB-YP382265SUQ
CSB-EP382265SUQ
CSB-BP382265SUQ
CSB-MP382265SUQ
CSB-EP382265SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 DNA mismatch repair protein MutS (mutS), partial CSB-YP382266SUQ
CSB-EP382266SUQ
CSB-BP382266SUQ
CSB-MP382266SUQ
CSB-EP382266SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Arginine--tRNA ligase (argS), partial CSB-YP382267SUQ
CSB-EP382267SUQ
CSB-BP382267SUQ
CSB-MP382267SUQ
CSB-EP382267SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L33 3 (rpmG3) CSB-YP382268SUQ
CSB-EP382268SUQ
CSB-BP382268SUQ
CSB-MP382268SUQ
CSB-EP382268SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP382269SUQ
CSB-EP382269SUQ
CSB-BP382269SUQ
CSB-MP382269SUQ
CSB-EP382269SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 DNA replication and repair protein recF (recF) CSB-YP382270SUQ
CSB-EP382270SUQ
CSB-BP382270SUQ
CSB-MP382270SUQ
CSB-EP382270SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP382271LND
CSB-EP382271LND
CSB-BP382271LND
CSB-MP382271LND
CSB-EP382271LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP382272LND
CSB-EP382272LND
CSB-BP382272LND
CSB-MP382272LND
CSB-EP382272LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Phosphate acyltransferase (plsX) CSB-YP382273LND
CSB-EP382273LND
CSB-BP382273LND
CSB-MP382273LND
CSB-EP382273LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Ribosomal protein L11 methyltransferase (prmA) CSB-YP382274LND
CSB-EP382274LND
CSB-BP382274LND
CSB-MP382274LND
CSB-EP382274LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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