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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus 3-dehydroquinate synthase (aroB) CSB-YP383197PZF
CSB-EP383197PZF
CSB-BP383197PZF
CSB-MP383197PZF
CSB-EP383197PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP383198PZF
CSB-EP383198PZF
CSB-BP383198PZF
CSB-MP383198PZF
CSB-EP383198PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center Psb28 protein (psb28) CSB-YP383199PZF
CSB-EP383199PZF
CSB-BP383199PZF
CSB-MP383199PZF
CSB-EP383199PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP383200PZF
CSB-EP383200PZF
CSB-BP383200PZF
CSB-MP383200PZF
CSB-EP383200PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Threonine--tRNA ligase (thrS), partial CSB-YP383201PZF
CSB-EP383201PZF
CSB-BP383201PZF
CSB-MP383201PZF
CSB-EP383201PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 5'-nucleotidase surE (surE) CSB-YP383202PZF
CSB-EP383202PZF
CSB-BP383202PZF
CSB-MP383202PZF
CSB-EP383202PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Indole-3-glycerol phosphate synthase (trpC) CSB-YP383203PZF
CSB-EP383203PZF
CSB-BP383203PZF
CSB-MP383203PZF
CSB-EP383203PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus PKHD-type hydroxylase P9303_20491 (P9303_20491) CSB-YP383204PZF
CSB-EP383204PZF
CSB-BP383204PZF
CSB-MP383204PZF
CSB-EP383204PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Putative membrane protein insertion efficiency factor (P9303_21151) CSB-YP383205PZF
CSB-EP383205PZF
CSB-BP383205PZF
CSB-MP383205PZF
CSB-EP383205PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP383206PZF
CSB-EP383206PZF
CSB-BP383206PZF
CSB-MP383206PZF
CSB-EP383206PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP383207PZF1
CSB-EP383207PZF1
CSB-BP383207PZF1
CSB-MP383207PZF1
CSB-EP383207PZF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 15,16-dihydrobiliverdin:ferredoxin oxidoreductase (pebA) CSB-YP383208PZF
CSB-EP383208PZF
CSB-BP383208PZF
CSB-MP383208PZF
CSB-EP383208PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional pantoate ligase/cytidylate kinase (panC/cmk) CSB-YP383209PZF
CSB-EP383209PZF
CSB-BP383209PZF
CSB-MP383209PZF
CSB-EP383209PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit N CSB-YP383210PZF
CSB-EP383210PZF
CSB-BP383210PZF
CSB-MP383210PZF
CSB-EP383210PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L2 (rplB) CSB-YP383211PZF
CSB-EP383211PZF
CSB-BP383211PZF
CSB-MP383211PZF
CSB-EP383211PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S3 (rpsC) CSB-YP383212PZF
CSB-EP383212PZF
CSB-BP383212PZF
CSB-MP383212PZF
CSB-EP383212PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S17 (rpsQ) CSB-YP383213PZF
CSB-EP383213PZF
CSB-BP383213PZF
CSB-MP383213PZF
CSB-EP383213PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L18 (rplR) CSB-YP383214PZF
CSB-EP383214PZF
CSB-BP383214PZF
CSB-MP383214PZF
CSB-EP383214PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I P700 chlorophyll a apoprotein A2 (psaB), partial CSB-YP383215PZF
CSB-EP383215PZF
CSB-BP383215PZF
CSB-MP383215PZF
CSB-EP383215PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S7 (rpsG) CSB-YP383216PZF
CSB-EP383216PZF
CSB-BP383216PZF
CSB-MP383216PZF
CSB-EP383216PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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