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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Ribose-5-phosphate isomerase A (rpiA) CSB-YP383177PZF
CSB-EP383177PZF
CSB-BP383177PZF
CSB-MP383177PZF
CSB-EP383177PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP383178PZF
CSB-EP383178PZF
CSB-BP383178PZF
CSB-MP383178PZF
CSB-EP383178PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP383179PZF
CSB-EP383179PZF
CSB-BP383179PZF
CSB-MP383179PZF
CSB-EP383179PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP synthase gamma chain (atpG) CSB-YP383180PZF
CSB-EP383180PZF
CSB-BP383180PZF
CSB-MP383180PZF
CSB-EP383180PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP383181PZF
CSB-EP383181PZF
CSB-BP383181PZF
CSB-MP383181PZF
CSB-EP383181PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable transcriptional regulatory protein P9303_05381 (P9303_05381) CSB-YP383182PZF
CSB-EP383182PZF
CSB-BP383182PZF
CSB-MP383182PZF
CSB-EP383182PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Circadian clock protein kaiB (kaiB) CSB-YP383183PZF
CSB-EP383183PZF
CSB-BP383183PZF
CSB-MP383183PZF
CSB-EP383183PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit 2, partial CSB-YP383184PZF
CSB-EP383184PZF
CSB-BP383184PZF
CSB-MP383184PZF
CSB-EP383184PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 60 kDa chaperonin 2 (groL2), partial CSB-YP383185PZF
CSB-EP383185PZF
CSB-BP383185PZF
CSB-MP383185PZF
CSB-EP383185PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex iron-sulfur subunit (petC), partial CSB-YP383186PZF1
CSB-EP383186PZF1
CSB-BP383186PZF1
CSB-MP383186PZF1
CSB-EP383186PZF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP383187PZF
CSB-EP383187PZF
CSB-BP383187PZF
CSB-MP383187PZF
CSB-EP383187PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adenylosuccinate synthetase (purA) CSB-YP383188PZF
CSB-EP383188PZF
CSB-BP383188PZF
CSB-MP383188PZF
CSB-EP383188PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (chlL) CSB-YP383189PZF
CSB-EP383189PZF
CSB-BP383189PZF
CSB-MP383189PZF
CSB-EP383189PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP383190PZF
CSB-EP383190PZF
CSB-BP383190PZF
CSB-MP383190PZF
CSB-EP383190PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP383191PZF1
CSB-EP383191PZF1
CSB-BP383191PZF1
CSB-MP383191PZF1
CSB-EP383191PZF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP383192PZF
CSB-EP383192PZF
CSB-BP383192PZF
CSB-MP383192PZF
CSB-EP383192PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP383193PZF
CSB-EP383193PZF
CSB-BP383193PZF
CSB-MP383193PZF
CSB-EP383193PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S21 (rpsU) CSB-YP383194PZF
CSB-EP383194PZF
CSB-BP383194PZF
CSB-MP383194PZF
CSB-EP383194PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0182 protein P9303_14611 (P9303_14611), partial CSB-YP383195PZF
CSB-EP383195PZF
CSB-BP383195PZF
CSB-MP383195PZF
CSB-EP383195PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphopantetheine adenylyltransferase (coaD) CSB-YP383196PZF
CSB-EP383196PZF
CSB-BP383196PZF
CSB-MP383196PZF
CSB-EP383196PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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