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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus UPF0367 protein P9301_01411 (P9301_01411) CSB-YP384597PZE
CSB-EP384597PZE
CSB-BP384597PZE
CSB-MP384597PZE
CSB-EP384597PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase chain 4, partial CSB-YP384598PZE
CSB-EP384598PZE
CSB-BP384598PZE
CSB-MP384598PZE
CSB-EP384598PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Tryptophan synthase beta chain (trpB) CSB-YP384599PZE
CSB-EP384599PZE
CSB-BP384599PZE
CSB-MP384599PZE
CSB-EP384599PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable rRNA maturation factor (P9301_02021) CSB-YP384600PZE
CSB-EP384600PZE
CSB-BP384600PZE
CSB-MP384600PZE
CSB-EP384600PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L7/L12 (rplL) CSB-YP384601PZE
CSB-EP384601PZE
CSB-BP384601PZE
CSB-MP384601PZE
CSB-EP384601PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable aspartoacylase (P9301_02431) CSB-YP384602PZE
CSB-EP384602PZE
CSB-BP384602PZE
CSB-MP384602PZE
CSB-EP384602PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chorismate synthase (aroC) CSB-YP384603PZE
CSB-EP384603PZE
CSB-BP384603PZE
CSB-MP384603PZE
CSB-EP384603PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoglucosamine mutase (glmM) CSB-YP384604PZE
CSB-EP384604PZE
CSB-BP384604PZE
CSB-MP384604PZE
CSB-EP384604PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein H (psbH), partial CSB-YP384605PZE1
CSB-EP384605PZE1
CSB-BP384605PZE1
CSB-MP384605PZE1
CSB-EP384605PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus CinA-like protein (P9301_02801) CSB-YP384606PZE
CSB-EP384606PZE
CSB-BP384606PZE
CSB-MP384606PZE
CSB-EP384606PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP384607PZE
CSB-EP384607PZE
CSB-BP384607PZE
CSB-MP384607PZE
CSB-EP384607PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit K CSB-YP384608PZE
CSB-EP384608PZE
CSB-BP384608PZE
CSB-MP384608PZE
CSB-EP384608PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b559 subunit alpha (psbE), partial CSB-YP384609PZE1
CSB-EP384609PZE1
CSB-BP384609PZE1
CSB-MP384609PZE1
CSB-EP384609PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus S-adenosylmethionine synthase (metK) CSB-YP384610PZE
CSB-EP384610PZE
CSB-BP384610PZE
CSB-MP384610PZE
CSB-EP384610PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP384611PZE
CSB-EP384611PZE
CSB-BP384611PZE
CSB-MP384611PZE
CSB-EP384611PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-dehydroquinate dehydratase (aroQ) CSB-YP384612PZE
CSB-EP384612PZE
CSB-BP384612PZE
CSB-MP384612PZE
CSB-EP384612PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Putative membrane protein insertion efficiency factor (P9301_04301) CSB-YP384613PZE
CSB-EP384613PZE
CSB-BP384613PZE
CSB-MP384613PZE
CSB-EP384613PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Elongation factor 4 (lepA), partial CSB-YP384614PZE
CSB-EP384614PZE
CSB-BP384614PZE
CSB-MP384614PZE
CSB-EP384614PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0284 protein P9301_04631 (P9301_04631) CSB-YP384615PZE
CSB-EP384615PZE
CSB-BP384615PZE
CSB-MP384615PZE
CSB-EP384615PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP384616PZE
CSB-EP384616PZE
CSB-BP384616PZE
CSB-MP384616PZE
CSB-EP384616PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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