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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium thermocellum 30S ribosomal protein S4 (rpsD) CSB-YP384037DUT
CSB-EP384037DUT
CSB-BP384037DUT
CSB-MP384037DUT
CSB-EP384037DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP384038DUT
CSB-EP384038DUT
CSB-BP384038DUT
CSB-MP384038DUT
CSB-EP384038DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Sugar fermentation stimulation protein homolog (sfsA) CSB-YP384039DUT
CSB-EP384039DUT
CSB-BP384039DUT
CSB-MP384039DUT
CSB-EP384039DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP384040DUT
CSB-EP384040DUT
CSB-BP384040DUT
CSB-MP384040DUT
CSB-EP384040DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Diaminopimelate epimerase (dapF) CSB-YP384041DUT
CSB-EP384041DUT
CSB-BP384041DUT
CSB-MP384041DUT
CSB-EP384041DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Alanine--tRNA ligase (alaS), partial CSB-YP384042DUT
CSB-EP384042DUT
CSB-BP384042DUT
CSB-MP384042DUT
CSB-EP384042DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Sulfate adenylyltransferase (sat) CSB-YP384043SUP
CSB-EP384043SUP
CSB-BP384043SUP
CSB-MP384043SUP
CSB-EP384043SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP384044SUP
CSB-EP384044SUP
CSB-BP384044SUP
CSB-MP384044SUP
CSB-EP384044SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP384045SUP
CSB-EP384045SUP
CSB-BP384045SUP
CSB-MP384045SUP
CSB-EP384045SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Orotate phosphoribosyltransferase (pyrE) CSB-YP384046SUP
CSB-EP384046SUP
CSB-BP384046SUP
CSB-MP384046SUP
CSB-EP384046SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Probable thymidylate kinase (tmk) CSB-YP384047SUP
CSB-EP384047SUP
CSB-BP384047SUP
CSB-MP384047SUP
CSB-EP384047SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 50S ribosomal protein L40e (rpl40e) CSB-YP384048SUP
CSB-EP384048SUP
CSB-BP384048SUP
CSB-MP384048SUP
CSB-EP384048SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Elongation factor 1-beta (ef1b) CSB-YP384049SUP
CSB-EP384049SUP
CSB-BP384049SUP
CSB-MP384049SUP
CSB-EP384049SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Signal recognition 54 kDa protein (srp54) CSB-YP384050SUP
CSB-EP384050SUP
CSB-BP384050SUP
CSB-MP384050SUP
CSB-EP384050SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Uridylate kinase (pyrH) CSB-YP384051SUP
CSB-EP384051SUP
CSB-BP384051SUP
CSB-MP384051SUP
CSB-EP384051SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 30S ribosomal protein S12P (rps12p) CSB-YP384052SUP
CSB-EP384052SUP
CSB-BP384052SUP
CSB-MP384052SUP
CSB-EP384052SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus 30S ribosomal protein S4P (rps4p) CSB-YP384053SUP
CSB-EP384053SUP
CSB-BP384053SUP
CSB-MP384053SUP
CSB-EP384053SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Glutamate--tRNA ligase (gltX), partial CSB-YP384054SUP
CSB-EP384054SUP
CSB-BP384054SUP
CSB-MP384054SUP
CSB-EP384054SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus UPF0095 protein Smar_0889 (Smar_0889) CSB-YP384055SUP
CSB-EP384055SUP
CSB-BP384055SUP
CSB-MP384055SUP
CSB-EP384055SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus tRNA (cytidine (56)-2'-O)-methyltransferase CSB-YP384056SUP
CSB-EP384056SUP
CSB-BP384056SUP
CSB-MP384056SUP
CSB-EP384056SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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