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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanoculleus marisnigri UPF0212 protein Memar_0858 (Memar_0858) CSB-YP385037MNU
CSB-EP385037MNU
CSB-BP385037MNU
CSB-MP385037MNU
CSB-EP385037MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP385038MNU
CSB-EP385038MNU
CSB-BP385038MNU
CSB-MP385038MNU
CSB-EP385038MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Uridylate kinase (pyrH) CSB-YP385039MNU
CSB-EP385039MNU
CSB-BP385039MNU
CSB-MP385039MNU
CSB-EP385039MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP385040MNU
CSB-EP385040MNU
CSB-BP385040MNU
CSB-MP385040MNU
CSB-EP385040MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Replication factor C small subunit (rfcS) CSB-YP385041MNU
CSB-EP385041MNU
CSB-BP385041MNU
CSB-MP385041MNU
CSB-EP385041MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Proteasome-activating nucleotidase (pan) CSB-YP385042MNU
CSB-EP385042MNU
CSB-BP385042MNU
CSB-MP385042MNU
CSB-EP385042MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Dihydrodipicolinate reductase (dapB) CSB-YP385043MNU
CSB-EP385043MNU
CSB-BP385043MNU
CSB-MP385043MNU
CSB-EP385043MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP385044MNU
CSB-EP385044MNU
CSB-BP385044MNU
CSB-MP385044MNU
CSB-EP385044MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri tRNA (cytidine (56)-2'-O)-methyltransferase CSB-YP385045MNU
CSB-EP385045MNU
CSB-BP385045MNU
CSB-MP385045MNU
CSB-EP385045MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Histidine--tRNA ligase (hisS) CSB-YP385046MNU
CSB-EP385046MNU
CSB-BP385046MNU
CSB-MP385046MNU
CSB-EP385046MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Ribonuclease P protein component 2 (rnp2) CSB-YP385047MNU
CSB-EP385047MNU
CSB-BP385047MNU
CSB-MP385047MNU
CSB-EP385047MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP385048MNU
CSB-EP385048MNU
CSB-BP385048MNU
CSB-MP385048MNU
CSB-EP385048MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri DNA-directed RNA polymerase subunit N (rpoN) CSB-YP385049MNU
CSB-EP385049MNU
CSB-BP385049MNU
CSB-MP385049MNU
CSB-EP385049MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 30S ribosomal protein S2P (rps2p) CSB-YP385050MNU
CSB-EP385050MNU
CSB-BP385050MNU
CSB-MP385050MNU
CSB-EP385050MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP385051MNU
CSB-EP385051MNU
CSB-BP385051MNU
CSB-MP385051MNU
CSB-EP385051MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri DNA ligase (lig), partial CSB-YP385052MNU
CSB-EP385052MNU
CSB-BP385052MNU
CSB-MP385052MNU
CSB-EP385052MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri DNA repair and recombination protein radA (radA) CSB-YP385053MNU
CSB-EP385053MNU
CSB-BP385053MNU
CSB-MP385053MNU
CSB-EP385053MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Prefoldin subunit alpha (pfdA) CSB-YP385054MNU
CSB-EP385054MNU
CSB-BP385054MNU
CSB-MP385054MNU
CSB-EP385054MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri 50S ribosomal protein L31e (rpl31e) CSB-YP385055MNU
CSB-EP385055MNU
CSB-BP385055MNU
CSB-MP385055MNU
CSB-EP385055MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Probable cobyric acid synthase (cobQ) CSB-YP385056MNU
CSB-EP385056MNU
CSB-BP385056MNU
CSB-MP385056MNU
CSB-EP385056MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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