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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pyrobaculum calidifontis DNA ligase (lig), partial CSB-YP384298PZV
CSB-EP384298PZV
CSB-BP384298PZV
CSB-MP384298PZV
CSB-EP384298PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis ATP phosphoribosyltransferase (hisG) CSB-YP384299PZV
CSB-EP384299PZV
CSB-BP384299PZV
CSB-MP384299PZV
CSB-EP384299PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Sugar fermentation stimulation protein homolog (sfsA) CSB-YP384300PZV
CSB-EP384300PZV
CSB-BP384300PZV
CSB-MP384300PZV
CSB-EP384300PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S8P (rps8p) CSB-YP384301PZV
CSB-EP384301PZV
CSB-BP384301PZV
CSB-MP384301PZV
CSB-EP384301PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0248 protein Pcal_0252 (Pcal_0252) CSB-YP384302PZV
CSB-EP384302PZV
CSB-BP384302PZV
CSB-MP384302PZV
CSB-EP384302PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S10P (rps10p) CSB-YP384303PZV
CSB-EP384303PZV
CSB-BP384303PZV
CSB-MP384303PZV
CSB-EP384303PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Translation initiation factor 5A (eIF5A) CSB-YP384304PZV
CSB-EP384304PZV
CSB-BP384304PZV
CSB-MP384304PZV
CSB-EP384304PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP384305PZV
CSB-EP384305PZV
CSB-BP384305PZV
CSB-MP384305PZV
CSB-EP384305PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S19P (rps19p) CSB-YP384306PZV
CSB-EP384306PZV
CSB-BP384306PZV
CSB-MP384306PZV
CSB-EP384306PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Arginine decarboxylase proenzyme (Pcal_0636) CSB-YP384307PZV
CSB-EP384307PZV
CSB-BP384307PZV
CSB-MP384307PZV
CSB-EP384307PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Leucine--tRNA ligase (leuS), partial CSB-YP384308PZV
CSB-EP384308PZV
CSB-BP384308PZV
CSB-MP384308PZV
CSB-EP384308PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 50S ribosomal protein L30P (rpl30p) CSB-YP384309PZV
CSB-EP384309PZV
CSB-BP384309PZV
CSB-MP384309PZV
CSB-EP384309PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Chromatin protein Cren7 (creN7) CSB-YP384310PZV
CSB-EP384310PZV
CSB-BP384310PZV
CSB-MP384310PZV
CSB-EP384310PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP384311PZV
CSB-EP384311PZV
CSB-BP384311PZV
CSB-MP384311PZV
CSB-EP384311PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis DNA/RNA-binding protein Alba (albA) CSB-YP384312PZV
CSB-EP384312PZV
CSB-BP384312PZV
CSB-MP384312PZV
CSB-EP384312PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 50S ribosomal protein L39e (rpl39e) CSB-YP384313PZV
CSB-EP384313PZV
CSB-BP384313PZV
CSB-MP384313PZV
CSB-EP384313PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis UPF0095 protein Pcal_1050 (Pcal_1050) CSB-YP384314PZV
CSB-EP384314PZV
CSB-BP384314PZV
CSB-MP384314PZV
CSB-EP384314PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Ribonuclease Z (rnz) CSB-YP384315PZV
CSB-EP384315PZV
CSB-BP384315PZV
CSB-MP384315PZV
CSB-EP384315PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 50S ribosomal protein L34e (rpl34e) CSB-YP384316PZV
CSB-EP384316PZV
CSB-BP384316PZV
CSB-MP384316PZV
CSB-EP384316PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S15P/S13e (rps15p) CSB-YP384317PZV
CSB-EP384317PZV
CSB-BP384317PZV
CSB-MP384317PZV
CSB-EP384317PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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