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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrobaculum calidifontis Proline--tRNA ligase (proS) CSB-YP385497PZV
CSB-EP385497PZV
CSB-BP385497PZV
CSB-MP385497PZV
CSB-EP385497PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Glutamyl-tRNA (Gln) amidotransferase subunit E, partial CSB-YP385498PZV
CSB-EP385498PZV
CSB-BP385498PZV
CSB-MP385498PZV
CSB-EP385498PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Elongation factor 1-alpha (tuf) CSB-YP385499PZV
CSB-EP385499PZV
CSB-BP385499PZV
CSB-MP385499PZV
CSB-EP385499PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Phosphoenolpyruvate carboxylase (ppcA) CSB-YP385500PZV
CSB-EP385500PZV
CSB-BP385500PZV
CSB-MP385500PZV
CSB-EP385500PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Cobalamin synthase (cobS), partial CSB-YP385501PZV1
CSB-EP385501PZV1
CSB-BP385501PZV1
CSB-MP385501PZV1
CSB-EP385501PZV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP385502PZV
CSB-EP385502PZV
CSB-BP385502PZV
CSB-MP385502PZV
CSB-EP385502PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 30S ribosomal protein S4e (rps4e) CSB-YP385503PZV
CSB-EP385503PZV
CSB-BP385503PZV
CSB-MP385503PZV
CSB-EP385503PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP385504PZV
CSB-EP385504PZV
CSB-BP385504PZV
CSB-MP385504PZV
CSB-EP385504PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP385505PZV
CSB-EP385505PZV
CSB-BP385505PZV
CSB-MP385505PZV
CSB-EP385505PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Serine--tRNA ligase (serS) CSB-YP385506PZV
CSB-EP385506PZV
CSB-BP385506PZV
CSB-MP385506PZV
CSB-EP385506PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Tryptophan--tRNA ligase (trpS) CSB-YP385507PZV
CSB-EP385507PZV
CSB-BP385507PZV
CSB-MP385507PZV
CSB-EP385507PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Prefoldin subunit alpha (pfdA) CSB-YP385508PZV
CSB-EP385508PZV
CSB-BP385508PZV
CSB-MP385508PZV
CSB-EP385508PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP385509PZV
CSB-EP385509PZV
CSB-BP385509PZV
CSB-MP385509PZV
CSB-EP385509PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Bifunctional protein FolD (folD) CSB-YP385510PZV
CSB-EP385510PZV
CSB-BP385510PZV
CSB-MP385510PZV
CSB-EP385510PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Proteasome subunit beta 2 (psmB2) CSB-YP385511PZV
CSB-EP385511PZV
CSB-BP385511PZV
CSB-MP385511PZV
CSB-EP385511PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis DNA repair and recombination protein radA (radA) CSB-YP385512PZV
CSB-EP385512PZV
CSB-BP385512PZV
CSB-MP385512PZV
CSB-EP385512PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis V-type ATP synthase alpha chain (atpA), partial CSB-YP385513PZV
CSB-EP385513PZV
CSB-BP385513PZV
CSB-MP385513PZV
CSB-EP385513PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis DNA-directed RNA polymerase subunit N (rpoN) CSB-YP385514PZV
CSB-EP385514PZV
CSB-BP385514PZV
CSB-MP385514PZV
CSB-EP385514PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP385515PZV
CSB-EP385515PZV
CSB-BP385515PZV
CSB-MP385515PZV
CSB-EP385515PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum calidifontis Aspartate carbamoyltransferase (pyrB) CSB-YP385516PZV
CSB-EP385516PZV
CSB-BP385516PZV
CSB-MP385516PZV
CSB-EP385516PZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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