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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 5b Adenylosuccinate synthetase (purA) CSB-YP384377AUF
CSB-EP384377AUF
CSB-BP384377AUF
CSB-MP384377AUF
CSB-EP384377AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP384378AUF
CSB-EP384378AUF
CSB-BP384378AUF
CSB-MP384378AUF
CSB-EP384378AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Beta-hexosaminidase (nagZ) CSB-YP384379AUF
CSB-EP384379AUF
CSB-BP384379AUF
CSB-MP384379AUF
CSB-EP384379AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Protein CyaY (cyaY) CSB-YP384380AUF
CSB-EP384380AUF
CSB-BP384380AUF
CSB-MP384380AUF
CSB-EP384380AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Phosphopantetheine adenylyltransferase (coaD) CSB-YP384381AUF
CSB-EP384381AUF
CSB-BP384381AUF
CSB-MP384381AUF
CSB-EP384381AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Shikimate dehydrogenase (aroE) CSB-YP384382AUF
CSB-EP384382AUF
CSB-BP384382AUF
CSB-MP384382AUF
CSB-EP384382AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Anthranilate phosphoribosyltransferase (trpD) CSB-YP384383AUF
CSB-EP384383AUF
CSB-BP384383AUF
CSB-MP384383AUF
CSB-EP384383AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 30S ribosomal protein S6 (rpsF) CSB-YP384384AUF
CSB-EP384384AUF
CSB-BP384384AUF
CSB-MP384384AUF
CSB-EP384384AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Translation initiation factor IF-1 (infA) CSB-YP384385AUF
CSB-EP384385AUF
CSB-BP384385AUF
CSB-MP384385AUF
CSB-EP384385AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP384386AUF
CSB-EP384386AUF
CSB-BP384386AUF
CSB-MP384386AUF
CSB-EP384386AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Arginine repressor (argR) CSB-YP384387AUF
CSB-EP384387AUF
CSB-BP384387AUF
CSB-MP384387AUF
CSB-EP384387AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP384388AUF1
CSB-EP384388AUF1
CSB-BP384388AUF1
CSB-MP384388AUF1
CSB-EP384388AUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Thiol:disulfide interchange protein DsbD (dsbD), partial CSB-YP384389AUF
CSB-EP384389AUF
CSB-BP384389AUF
CSB-MP384389AUF
CSB-EP384389AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Probable oxaloacetate decarboxylase gamma chain (oadG), partial CSB-YP384390AUF1
CSB-EP384390AUF1
CSB-BP384390AUF1
CSB-MP384390AUF1
CSB-EP384390AUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribose-5-phosphate isomerase A (rpiA) CSB-YP384391AUF
CSB-EP384391AUF
CSB-BP384391AUF
CSB-MP384391AUF
CSB-EP384391AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP384392AUF
CSB-EP384392AUF
CSB-BP384392AUF
CSB-MP384392AUF
CSB-EP384392AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Protein-export protein secB (secB) CSB-YP384393AUF
CSB-EP384393AUF
CSB-BP384393AUF
CSB-MP384393AUF
CSB-EP384393AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Pantothenate kinase (coaA) CSB-YP384394AUF
CSB-EP384394AUF
CSB-BP384394AUF
CSB-MP384394AUF
CSB-EP384394AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Fumarate reductase subunit D (frdD), partial CSB-YP384395AUF1
CSB-EP384395AUF1
CSB-BP384395AUF1
CSB-MP384395AUF1
CSB-EP384395AUF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribosomal protein L11 methyltransferase (prmA) CSB-YP384396AUF
CSB-EP384396AUF
CSB-BP384396AUF
CSB-MP384396AUF
CSB-EP384396AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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