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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus 30S ribosomal protein S4 (rpsD) CSB-YP385797PZE
CSB-EP385797PZE
CSB-BP385797PZE
CSB-MP385797PZE
CSB-EP385797PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 60 kDa chaperonin 1 (groL1), partial CSB-YP385798PZE
CSB-EP385798PZE
CSB-BP385798PZE
CSB-MP385798PZE
CSB-EP385798PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Apocytochrome f (petA), partial CSB-YP385799PZE1
CSB-EP385799PZE1
CSB-BP385799PZE1
CSB-MP385799PZE1
CSB-EP385799PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0234 protein P9301_05061 (P9301_05061) CSB-YP385800PZE
CSB-EP385800PZE
CSB-BP385800PZE
CSB-MP385800PZE
CSB-EP385800PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetylglutamate kinase (argB) CSB-YP385801PZE
CSB-EP385801PZE
CSB-BP385801PZE
CSB-MP385801PZE
CSB-EP385801PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Proline--tRNA ligase (proS), partial CSB-YP385802PZE
CSB-EP385802PZE
CSB-BP385802PZE
CSB-MP385802PZE
CSB-EP385802PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP385803PZE
CSB-EP385803PZE
CSB-BP385803PZE
CSB-MP385803PZE
CSB-EP385803PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit L, partial CSB-YP385804PZE1
CSB-EP385804PZE1
CSB-BP385804PZE1
CSB-MP385804PZE1
CSB-EP385804PZE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Gamma-glutamyl phosphate reductase (proA) CSB-YP385805PZE
CSB-EP385805PZE
CSB-BP385805PZE
CSB-MP385805PZE
CSB-EP385805PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP385806PZE
CSB-EP385806PZE
CSB-BP385806PZE
CSB-MP385806PZE
CSB-EP385806PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Histidine--tRNA ligase (hisS) CSB-YP385807PZE
CSB-EP385807PZE
CSB-BP385807PZE
CSB-MP385807PZE
CSB-EP385807PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP385808PZE
CSB-EP385808PZE
CSB-BP385808PZE
CSB-MP385808PZE
CSB-EP385808PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Putative Holliday junction resolvase (P9301_08521) CSB-YP385809PZE
CSB-EP385809PZE
CSB-BP385809PZE
CSB-MP385809PZE
CSB-EP385809PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease accessory protein UreG (ureG) CSB-YP385810PZE
CSB-EP385810PZE
CSB-BP385810PZE
CSB-MP385810PZE
CSB-EP385810PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease accessory protein UreD (ureD) CSB-YP385811PZE
CSB-EP385811PZE
CSB-BP385811PZE
CSB-MP385811PZE
CSB-EP385811PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease subunit alpha (ureC), partial CSB-YP385812PZE
CSB-EP385812PZE
CSB-BP385812PZE
CSB-MP385812PZE
CSB-EP385812PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Anthranilate phosphoribosyltransferase (trpD) CSB-YP385813PZE
CSB-EP385813PZE
CSB-BP385813PZE
CSB-MP385813PZE
CSB-EP385813PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP385814PZE
CSB-EP385814PZE
CSB-BP385814PZE
CSB-MP385814PZE
CSB-EP385814PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP385815PZE
CSB-EP385815PZE
CSB-BP385815PZE
CSB-MP385815PZE
CSB-EP385815PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S18 (rpsR) CSB-YP385816PZE
CSB-EP385816PZE
CSB-BP385816PZE
CSB-MP385816PZE
CSB-EP385816PZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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