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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylothermus marinus 30S ribosomal protein S8e (rps8e) CSB-YP387598SUP
CSB-EP387598SUP
CSB-BP387598SUP
CSB-MP387598SUP
CSB-EP387598SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (flpA) CSB-YP387599SUP
CSB-EP387599SUP
CSB-BP387599SUP
CSB-MP387599SUP
CSB-EP387599SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus DNA ligase (lig), partial CSB-YP387600SUP
CSB-EP387600SUP
CSB-BP387600SUP
CSB-MP387600SUP
CSB-EP387600SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus tRNA (Ile2) 2-agmatinylcytidine synthetase TiaS CSB-YP387601SUP
CSB-EP387601SUP
CSB-BP387601SUP
CSB-MP387601SUP
CSB-EP387601SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP387602ILT
CSB-EP387602ILT
CSB-BP387602ILT
CSB-MP387602ILT
CSB-EP387602ILT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP387603ILT
CSB-EP387603ILT
CSB-BP387603ILT
CSB-MP387603ILT
CSB-EP387603ILT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP387604ILT
CSB-EP387604ILT
CSB-BP387604ILT
CSB-MP387604ILT
CSB-EP387604ILT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 2 CSB-YP387605ROG
CSB-EP387605ROG
CSB-BP387605ROG
CSB-MP387605ROG
CSB-EP387605ROG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pfiesteria piscicida Calmodulin CSB-YP387606PYJ
CSB-EP387606PYJ
CSB-BP387606PYJ
CSB-MP387606PYJ
CSB-EP387606PYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU4 Non-structural protein 3c (3c) CSB-YP387607BOT
CSB-EP387607BOT
CSB-BP387607BOT
CSB-MP387607BOT
CSB-EP387607BOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU4 Nucleoprotein (N) CSB-YP387608BOT
CSB-BP387608BOT
CSB-MP387608BOT
CSB-EP387608BOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU5 Non-structural protein 3b (3b) CSB-YP387609BOU
CSB-EP387609BOU
CSB-BP387609BOU
CSB-MP387609BOU
CSB-EP387609BOU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU5 Membrane protein (M), partial CSB-YP387610BOU1
CSB-EP387610BOU1
CSB-BP387610BOU1
CSB-MP387610BOU1
CSB-EP387610BOU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU9 Envelope small membrane protein (E), partial CSB-YP387611BOV1
CSB-EP387611BOV1
CSB-BP387611BOV1
CSB-MP387611BOV1
CSB-EP387611BOV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bat coronavirus HKU9 Non-structural protein 7b (7b) CSB-YP387612BOV
CSB-EP387612BOV
CSB-BP387612BOV
CSB-MP387612BOV
CSB-EP387612BOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lasiodora sp. U3-theraphotoxin-Lsp1a CSB-YP387613LLI
CSB-EP387613LLI
CSB-BP387613LLI
CSB-MP387613LLI
CSB-EP387613LLI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acyl homoserine lactonase (aiiA) CSB-YP387614BRN
CSB-EP387614BRN
CSB-BP387614BRN
CSB-MP387614BRN
CSB-EP387614BRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human hepatitis A virus genotype IA Genome polyprotein, partial CSB-YP387615HSN
CSB-EP387615HSN
CSB-BP387615HSN
CSB-MP387615HSN
CSB-EP387615HSN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Pre-rRNA-processing protein RIX1 (RIX1), partial CSB-YP387616SXM
CSB-EP387616SXM
CSB-BP387616SXM
CSB-MP387616SXM
CSB-EP387616SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Autophagy-related protein 18 (ATG18), partial CSB-YP387617SXM
CSB-EP387617SXM
CSB-BP387617SXM
CSB-MP387617SXM
CSB-EP387617SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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