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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium thermocellum Cysteine--tRNA ligase (cysS) CSB-YP386398DUT
CSB-EP386398DUT
CSB-BP386398DUT
CSB-MP386398DUT
CSB-EP386398DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Endoglucanase SS (celS), partial CSB-YP386399DUT
CSB-EP386399DUT
CSB-BP386399DUT
CSB-MP386399DUT
CSB-EP386399DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP386400DUT
CSB-EP386400DUT
CSB-BP386400DUT
CSB-MP386400DUT
CSB-EP386400DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Carbon storage regulator homolog (csrA) CSB-YP386401DUT
CSB-EP386401DUT
CSB-BP386401DUT
CSB-MP386401DUT
CSB-EP386401DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum S-adenosylmethionine synthase (metK) CSB-YP386402DUT
CSB-EP386402DUT
CSB-BP386402DUT
CSB-MP386402DUT
CSB-EP386402DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Undecaprenyl-diphosphatase (uppP), partial CSB-YP386403DUT
CSB-EP386403DUT
CSB-BP386403DUT
CSB-MP386403DUT
CSB-EP386403DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP386404DUT
CSB-EP386404DUT
CSB-BP386404DUT
CSB-MP386404DUT
CSB-EP386404DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L34 (rpmH) CSB-YP386405DUT
CSB-EP386405DUT
CSB-BP386405DUT
CSB-MP386405DUT
CSB-EP386405DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP386406DUT
CSB-EP386406DUT
CSB-BP386406DUT
CSB-MP386406DUT
CSB-EP386406DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP386407DUT
CSB-EP386407DUT
CSB-BP386407DUT
CSB-MP386407DUT
CSB-EP386407DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP386408DUT
CSB-EP386408DUT
CSB-BP386408DUT
CSB-MP386408DUT
CSB-EP386408DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Putative septation protein spoVG (spoVG) CSB-YP386409DUT
CSB-EP386409DUT
CSB-BP386409DUT
CSB-MP386409DUT
CSB-EP386409DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP386410DUT
CSB-EP386410DUT
CSB-BP386410DUT
CSB-MP386410DUT
CSB-EP386410DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S12 (rpsL) CSB-YP386411DUT
CSB-EP386411DUT
CSB-BP386411DUT
CSB-MP386411DUT
CSB-EP386411DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Trigger factor (tig) CSB-YP386412DUT
CSB-EP386412DUT
CSB-BP386412DUT
CSB-MP386412DUT
CSB-EP386412DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Endoglucanase C (celC) CSB-YP386413DUT
CSB-EP386413DUT
CSB-BP386413DUT
CSB-MP386413DUT
CSB-EP386413DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP386414DUT
CSB-EP386414DUT
CSB-BP386414DUT
CSB-MP386414DUT
CSB-EP386414DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP phosphoribosyltransferase (hisG) CSB-YP386415DUT
CSB-EP386415DUT
CSB-BP386415DUT
CSB-MP386415DUT
CSB-EP386415DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Transcription elongation factor GreA (greA) CSB-YP386416DUT
CSB-EP386416DUT
CSB-BP386416DUT
CSB-MP386416DUT
CSB-EP386416DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L22 (rplV) CSB-YP386417DUT
CSB-EP386417DUT
CSB-BP386417DUT
CSB-MP386417DUT
CSB-EP386417DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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