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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacter sp. Glutathione-regulated potassium-efflux system ancillary protein KefG (kefG) CSB-YP394724EIZ
CSB-EP394724EIZ
CSB-BP394724EIZ
CSB-MP394724EIZ
CSB-EP394724EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 3-dehydroquinate synthase (aroB) CSB-YP394725EIZ
CSB-EP394725EIZ
CSB-BP394725EIZ
CSB-MP394725EIZ
CSB-EP394725EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. HTH-type transcriptional regulator malT (malT), partial CSB-YP394726EIZ
CSB-EP394726EIZ
CSB-BP394726EIZ
CSB-MP394726EIZ
CSB-EP394726EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP394727EIZ
CSB-EP394727EIZ
CSB-BP394727EIZ
CSB-MP394727EIZ
CSB-EP394727EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP394728EIZ1
CSB-EP394728EIZ1
CSB-BP394728EIZ1
CSB-MP394728EIZ1
CSB-EP394728EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. ATP-dependent RNA helicase rhlB (rhlB) CSB-YP394729EIZ
CSB-EP394729EIZ
CSB-BP394729EIZ
CSB-MP394729EIZ
CSB-EP394729EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Acetylglutamate kinase (argB) CSB-YP394730EIZ
CSB-EP394730EIZ
CSB-BP394730EIZ
CSB-MP394730EIZ
CSB-EP394730EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L31 (rpmE) CSB-YP394731EIZ
CSB-EP394731EIZ
CSB-BP394731EIZ
CSB-MP394731EIZ
CSB-EP394731EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Regulator of ribonuclease activity A (rraA) CSB-YP394732EIZ
CSB-EP394732EIZ
CSB-BP394732EIZ
CSB-MP394732EIZ
CSB-EP394732EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. D-ribose pyranase (rbsD) CSB-YP394733EIZ
CSB-EP394733EIZ
CSB-BP394733EIZ
CSB-MP394733EIZ
CSB-EP394733EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. 50S ribosomal protein L34 (rpmH) CSB-YP394734EIZ
CSB-EP394734EIZ
CSB-BP394734EIZ
CSB-MP394734EIZ
CSB-EP394734EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP394735PZU
CSB-EP394735PZU
CSB-BP394735PZU
CSB-MP394735PZU
CSB-EP394735PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Probable tRNA pseudouridine synthase B (truB) CSB-YP394736PZU
CSB-EP394736PZU
CSB-BP394736PZU
CSB-MP394736PZU
CSB-EP394736PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP394737PZU
CSB-EP394737PZU
CSB-BP394737PZU
CSB-MP394737PZU
CSB-EP394737PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Leucine--tRNA ligase (leuS), partial CSB-YP394738PZU
CSB-EP394738PZU
CSB-BP394738PZU
CSB-MP394738PZU
CSB-EP394738PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S6e (rps6e) CSB-YP394739PZU
CSB-EP394739PZU
CSB-BP394739PZU
CSB-MP394739PZU
CSB-EP394739PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0219 protein Pars_0793 (Pars_0793) CSB-YP394740PZU
CSB-EP394740PZU
CSB-BP394740PZU
CSB-MP394740PZU
CSB-EP394740PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum UPF0173 metal-dependent hydrolase Pars_0810 (Pars_0810) CSB-YP394741PZU
CSB-EP394741PZU
CSB-BP394741PZU
CSB-MP394741PZU
CSB-EP394741PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Phosphoenolpyruvate carboxylase (ppcA) CSB-YP394742PZU
CSB-EP394742PZU
CSB-BP394742PZU
CSB-MP394742PZU
CSB-EP394742PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S24e (rps24e) CSB-YP394743PZU
CSB-EP394743PZU
CSB-BP394743PZU
CSB-MP394743PZU
CSB-EP394743PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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