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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter sp. Met repressor (metJ) CSB-YP395724EIZ
CSB-EP395724EIZ
CSB-BP395724EIZ
CSB-MP395724EIZ
CSB-EP395724EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. HTH-type transcriptional activator rhaS (rhaS) CSB-YP395725EIZ
CSB-EP395725EIZ
CSB-BP395725EIZ
CSB-MP395725EIZ
CSB-EP395725EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Probable GTP-binding protein EngB (engB) CSB-YP395726EIZ
CSB-EP395726EIZ
CSB-BP395726EIZ
CSB-MP395726EIZ
CSB-EP395726EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. Aspartate--ammonia ligase (asnA) CSB-YP395727EIZ
CSB-EP395727EIZ
CSB-BP395727EIZ
CSB-MP395727EIZ
CSB-EP395727EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. tRNA modification GTPase MnmE (mnmE) CSB-YP395728EIZ
CSB-EP395728EIZ
CSB-BP395728EIZ
CSB-MP395728EIZ
CSB-EP395728EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S17e (rps17e) CSB-YP395729PZU
CSB-EP395729PZU
CSB-BP395729PZU
CSB-MP395729PZU
CSB-EP395729PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum V-type ATP synthase beta chain (atpB) CSB-YP395730PZU
CSB-EP395730PZU
CSB-BP395730PZU
CSB-MP395730PZU
CSB-EP395730PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Translation initiation factor 2 subunit beta (eIF2B) CSB-YP395731PZU
CSB-EP395731PZU
CSB-BP395731PZU
CSB-MP395731PZU
CSB-EP395731PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Probable non-canonical purine NTP phosphatase (Pars_0872) CSB-YP395732PZU
CSB-EP395732PZU
CSB-BP395732PZU
CSB-MP395732PZU
CSB-EP395732PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 5'-nucleotidase surE (surE) CSB-YP395733PZU
CSB-EP395733PZU
CSB-BP395733PZU
CSB-MP395733PZU
CSB-EP395733PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Probable lipoyl synthase (lipA) CSB-YP395734PZU
CSB-EP395734PZU
CSB-BP395734PZU
CSB-MP395734PZU
CSB-EP395734PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Tryptophan synthase beta chain (trpB) CSB-YP395735PZU
CSB-EP395735PZU
CSB-BP395735PZU
CSB-MP395735PZU
CSB-EP395735PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Prefoldin subunit alpha (pfdA) CSB-YP395736PZU
CSB-EP395736PZU
CSB-BP395736PZU
CSB-MP395736PZU
CSB-EP395736PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Ketol-acid reductoisomerase (ilvC) CSB-YP395737PZU
CSB-EP395737PZU
CSB-BP395737PZU
CSB-MP395737PZU
CSB-EP395737PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 50S ribosomal protein L24e (rpl24e) CSB-YP395738PZU
CSB-EP395738PZU
CSB-BP395738PZU
CSB-MP395738PZU
CSB-EP395738PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Geranylgeranylglyceryl phosphate synthase (Pars_1793) CSB-YP395739PZU
CSB-EP395739PZU
CSB-BP395739PZU
CSB-MP395739PZU
CSB-EP395739PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum DNA-directed RNA polymerase subunit P (rpoP) CSB-YP395740PZU
CSB-EP395740PZU
CSB-BP395740PZU
CSB-MP395740PZU
CSB-EP395740PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S13P (rps13p) CSB-YP395741PZU
CSB-EP395741PZU
CSB-BP395741PZU
CSB-MP395741PZU
CSB-EP395741PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum 30S ribosomal protein S8P (rps8p) CSB-YP395742PZU
CSB-EP395742PZU
CSB-BP395742PZU
CSB-MP395742PZU
CSB-EP395742PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Bifunctional protein FolD (folD) CSB-YP395743PZU
CSB-EP395743PZU
CSB-BP395743PZU
CSB-MP395743PZU
CSB-EP395743PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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