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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodobacter sphaeroides Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP394784RLG
CSB-EP394784RLG
CSB-BP394784RLG
CSB-MP394784RLG
CSB-EP394784RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides LexA repressor (lexA) CSB-YP394785RLG
CSB-EP394785RLG
CSB-BP394785RLG
CSB-MP394785RLG
CSB-EP394785RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides GTPase obg (obg) CSB-YP394786RLG
CSB-EP394786RLG
CSB-BP394786RLG
CSB-MP394786RLG
CSB-EP394786RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Deoxycytidine triphosphate deaminase (dcd) CSB-YP394787STD
CSB-EP394787STD
CSB-BP394787STD
CSB-MP394787STD
CSB-EP394787STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Imidazolonepropionase (hutI) CSB-YP394788STD
CSB-EP394788STD
CSB-BP394788STD
CSB-MP394788STD
CSB-EP394788STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP394789STD
CSB-EP394789STD
CSB-BP394789STD
CSB-MP394789STD
CSB-EP394789STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 50S ribosomal protein L19 (rplS) CSB-YP394790STD
CSB-EP394790STD
CSB-BP394790STD
CSB-MP394790STD
CSB-EP394790STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP394791STD
CSB-EP394791STD
CSB-BP394791STD
CSB-MP394791STD
CSB-EP394791STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribosomal protein S12 methylthiotransferase RimO (rimO), partial CSB-YP394792STD
CSB-EP394792STD
CSB-BP394792STD
CSB-MP394792STD
CSB-EP394792STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Ribonuclease Y (rny), partial CSB-YP394793STD
CSB-EP394793STD
CSB-BP394793STD
CSB-MP394793STD
CSB-EP394793STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Alanine--tRNA ligase (alaS), partial CSB-YP394794STD
CSB-EP394794STD
CSB-BP394794STD
CSB-MP394794STD
CSB-EP394794STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 50S ribosomal protein L35 (rpmI) CSB-YP394795STD
CSB-EP394795STD
CSB-BP394795STD
CSB-MP394795STD
CSB-EP394795STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP394796STD
CSB-EP394796STD
CSB-BP394796STD
CSB-MP394796STD
CSB-EP394796STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Bifunctional protein FolD 1 (folD1) CSB-YP394797STD
CSB-EP394797STD
CSB-BP394797STD
CSB-MP394797STD
CSB-EP394797STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Carboxylate-amine ligase Strop_2542 (Strop_2542) CSB-YP394798STD
CSB-EP394798STD
CSB-BP394798STD
CSB-MP394798STD
CSB-EP394798STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP394799STD
CSB-EP394799STD
CSB-BP394799STD
CSB-MP394799STD
CSB-EP394799STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP394800STD
CSB-EP394800STD
CSB-BP394800STD
CSB-MP394800STD
CSB-EP394800STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Acetaldehyde dehydrogenase (Strop_3526) CSB-YP394801STD
CSB-EP394801STD
CSB-BP394801STD
CSB-MP394801STD
CSB-EP394801STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 50S ribosomal protein L31 (rpmE) CSB-YP394802STD
CSB-EP394802STD
CSB-BP394802STD
CSB-MP394802STD
CSB-EP394802STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP394803STD
CSB-EP394803STD
CSB-BP394803STD
CSB-MP394803STD
CSB-EP394803STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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