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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pseudomonas mendocina Urease accessory protein UreE (ureE) CSB-YP397946PZM
CSB-EP397946PZM
CSB-BP397946PZM
CSB-MP397946PZM
CSB-EP397946PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 3-dehydroquinate dehydratase (aroQ) CSB-YP397947PZM
CSB-EP397947PZM
CSB-BP397947PZM
CSB-MP397947PZM
CSB-EP397947PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP397948PZM
CSB-EP397948PZM
CSB-BP397948PZM
CSB-MP397948PZM
CSB-EP397948PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L13 (rplM) CSB-YP397949PZM
CSB-EP397949PZM
CSB-BP397949PZM
CSB-MP397949PZM
CSB-EP397949PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP397950PZM
CSB-EP397950PZM
CSB-BP397950PZM
CSB-MP397950PZM
CSB-EP397950PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP397951PZM
CSB-EP397951PZM
CSB-BP397951PZM
CSB-MP397951PZM
CSB-EP397951PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP397952PZM
CSB-EP397952PZM
CSB-BP397952PZM
CSB-MP397952PZM
CSB-EP397952PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Cell division topological specificity factor (minE) CSB-YP397953PZM
CSB-EP397953PZM
CSB-BP397953PZM
CSB-MP397953PZM
CSB-EP397953PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP397954PZM
CSB-EP397954PZM
CSB-BP397954PZM
CSB-MP397954PZM
CSB-EP397954PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Electron transport complex protein RnfA (rnfA), partial CSB-YP397955PZM1
CSB-EP397955PZM1
CSB-BP397955PZM1
CSB-MP397955PZM1
CSB-EP397955PZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Probable periplasmic serine endoprotease DegP-like (Pmen_1471) CSB-YP397956PZM
CSB-EP397956PZM
CSB-BP397956PZM
CSB-MP397956PZM
CSB-EP397956PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina tRNA (mo5U34)-methyltransferase CSB-YP397957PZM
CSB-EP397957PZM
CSB-BP397957PZM
CSB-MP397957PZM
CSB-EP397957PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Na (+)-translocating NADH-quinone reductase subunit B, partial CSB-YP397958PZM
CSB-EP397958PZM
CSB-BP397958PZM
CSB-MP397958PZM
CSB-EP397958PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Dihydrodipicolinate synthase (dapA) CSB-YP397959PZM
CSB-EP397959PZM
CSB-BP397959PZM
CSB-MP397959PZM
CSB-EP397959PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Cobalamin synthase (cobS), partial CSB-YP397960PZM1
CSB-EP397960PZM1
CSB-BP397960PZM1
CSB-MP397960PZM1
CSB-EP397960PZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Error-prone DNA polymerase (dnaE2), partial CSB-YP397961PZM
CSB-EP397961PZM
CSB-BP397961PZM
CSB-MP397961PZM
CSB-EP397961PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Trigger factor (tig) CSB-YP397962PZM
CSB-EP397962PZM
CSB-BP397962PZM
CSB-MP397962PZM
CSB-EP397962PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Elongation factor P (efp) CSB-YP397963PZM
CSB-EP397963PZM
CSB-BP397963PZM
CSB-MP397963PZM
CSB-EP397963PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Carboxylate-amine ligase Pmen_2301 (Pmen_2301) CSB-YP397964PZM
CSB-EP397964PZM
CSB-BP397964PZM
CSB-MP397964PZM
CSB-EP397964PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Putative arginyl-tRNA--protein transferase (ate) CSB-YP397965PZM
CSB-EP397965PZM
CSB-BP397965PZM
CSB-MP397965PZM
CSB-EP397965PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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