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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clavibacter michiganensis subsp. michiganensis N utilization substance protein B homolog (nusB) CSB-YP397926CWT
CSB-EP397926CWT
CSB-BP397926CWT
CSB-MP397926CWT
CSB-EP397926CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Glycerol kinase (glpK), partial CSB-YP397927CWT
CSB-EP397927CWT
CSB-BP397927CWT
CSB-MP397927CWT
CSB-EP397927CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Acetylglutamate kinase (argB) CSB-YP397928CWT
CSB-EP397928CWT
CSB-BP397928CWT
CSB-MP397928CWT
CSB-EP397928CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Ribosome-binding factor A (rbfA) CSB-YP397929CWT
CSB-EP397929CWT
CSB-BP397929CWT
CSB-MP397929CWT
CSB-EP397929CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 60 kDa chaperonin (groL), partial CSB-YP397930CWT
CSB-EP397930CWT
CSB-BP397930CWT
CSB-MP397930CWT
CSB-EP397930CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 10 kDa chaperonin (groS) CSB-YP397931CWT
CSB-EP397931CWT
CSB-BP397931CWT
CSB-MP397931CWT
CSB-EP397931CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 30S ribosomal protein S9 (rpsI) CSB-YP397932CWT
CSB-EP397932CWT
CSB-BP397932CWT
CSB-MP397932CWT
CSB-EP397932CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis 50S ribosomal protein L22 (rplV) CSB-YP397933CWT
CSB-EP397933CWT
CSB-BP397933CWT
CSB-MP397933CWT
CSB-EP397933CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP397934CWT
CSB-EP397934CWT
CSB-BP397934CWT
CSB-MP397934CWT
CSB-EP397934CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis UPF0336 protein CMM_2793 (CMM_2793) CSB-YP397935CWT
CSB-EP397935CWT
CSB-BP397935CWT
CSB-MP397935CWT
CSB-EP397935CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP397936CWT
CSB-EP397936CWT
CSB-BP397936CWT
CSB-MP397936CWT
CSB-EP397936CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP397937VDY
CSB-EP397937VDY
CSB-BP397937VDY
CSB-MP397937VDY
CSB-EP397937VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii ATP synthase epsilon chain (atpC) CSB-YP397938VDY
CSB-EP397938VDY
CSB-BP397938VDY
CSB-MP397938VDY
CSB-EP397938VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicomyosocius okutanii subsp. Calyptogena okutanii Bifunctional protein GlmU (glmU) CSB-YP397939VDY
CSB-EP397939VDY
CSB-BP397939VDY
CSB-MP397939VDY
CSB-EP397939VDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina UPF0149 protein Pmen_0324 (Pmen_0324) CSB-YP397940PZM
CSB-EP397940PZM
CSB-BP397940PZM
CSB-MP397940PZM
CSB-EP397940PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Sec-independent protein translocase protein TatA (tatA), partial CSB-YP397941PZM
CSB-EP397941PZM
CSB-BP397941PZM
CSB-MP397941PZM
CSB-EP397941PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP397942PZM
CSB-EP397942PZM
CSB-BP397942PZM
CSB-MP397942PZM
CSB-EP397942PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Cell volume regulation protein A homolog (cvrA), partial CSB-YP397943PZM
CSB-EP397943PZM
CSB-BP397943PZM
CSB-MP397943PZM
CSB-EP397943PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP397944PZM
CSB-EP397944PZM
CSB-BP397944PZM
CSB-MP397944PZM
CSB-EP397944PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 30S ribosomal protein S6 (rpsF) CSB-YP397945PZM
CSB-EP397945PZM
CSB-BP397945PZM
CSB-MP397945PZM
CSB-EP397945PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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