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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas mendocina LPS-assembly protein lptD (lptD), partial CSB-YP397986PZM
CSB-EP397986PZM
CSB-BP397986PZM
CSB-MP397986PZM
CSB-EP397986PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 30S ribosomal protein S21 (rpsU) CSB-YP397987PZM
CSB-EP397987PZM
CSB-BP397987PZM
CSB-MP397987PZM
CSB-EP397987PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Dihydroxy-acid dehydratase (ilvD), partial CSB-YP397988PZM
CSB-EP397988PZM
CSB-BP397988PZM
CSB-MP397988PZM
CSB-EP397988PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP397989PZM
CSB-EP397989PZM
CSB-BP397989PZM
CSB-MP397989PZM
CSB-EP397989PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Bifunctional protein GlmU (glmU) CSB-YP397990PZM
CSB-EP397990PZM
CSB-BP397990PZM
CSB-MP397990PZM
CSB-EP397990PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina 50S ribosomal protein L34 (rpmH) CSB-YP397991PZM
CSB-EP397991PZM
CSB-BP397991PZM
CSB-MP397991PZM
CSB-EP397991PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP397992STQ
CSB-EP397992STQ
CSB-BP397992STQ
CSB-MP397992STQ
CSB-EP397992STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP397993STQ
CSB-EP397993STQ
CSB-BP397993STQ
CSB-MP397993STQ
CSB-EP397993STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens FMN-dependent NADH-azoreductase (azoR) CSB-YP397994STQ
CSB-EP397994STQ
CSB-BP397994STQ
CSB-MP397994STQ
CSB-EP397994STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP397995STQ
CSB-EP397995STQ
CSB-BP397995STQ
CSB-MP397995STQ
CSB-EP397995STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP397996STQ
CSB-EP397996STQ
CSB-BP397996STQ
CSB-MP397996STQ
CSB-EP397996STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 50S ribosomal protein L9 (rplI) CSB-YP397997STQ
CSB-EP397997STQ
CSB-BP397997STQ
CSB-MP397997STQ
CSB-EP397997STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens S-ribosylhomocysteine lyase (luxS) CSB-YP397998STQ
CSB-EP397998STQ
CSB-BP397998STQ
CSB-MP397998STQ
CSB-EP397998STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP397999STQ
CSB-EP397999STQ
CSB-BP397999STQ
CSB-MP397999STQ
CSB-EP397999STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 30S ribosomal protein S20 (rpsT) CSB-YP398000STQ
CSB-EP398000STQ
CSB-BP398000STQ
CSB-MP398000STQ
CSB-EP398000STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Undecaprenyl-diphosphatase (uppP), partial CSB-YP398001STQ
CSB-EP398001STQ
CSB-BP398001STQ
CSB-MP398001STQ
CSB-EP398001STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Elongation factor 4 (lepA), partial CSB-YP398002STQ
CSB-EP398002STQ
CSB-BP398002STQ
CSB-MP398002STQ
CSB-EP398002STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens UPF0597 protein Sputcn32_1209 (Sputcn32_1209) CSB-YP398003STQ
CSB-EP398003STQ
CSB-BP398003STQ
CSB-MP398003STQ
CSB-EP398003STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens 30S ribosomal protein S2 (rpsB) CSB-YP398004STQ
CSB-EP398004STQ
CSB-BP398004STQ
CSB-MP398004STQ
CSB-EP398004STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Cysteine--tRNA ligase (cysS) CSB-YP398005STQ
CSB-EP398005STQ
CSB-BP398005STQ
CSB-MP398005STQ
CSB-EP398005STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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